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ELISA质控存在的问题探讨
引用本文:杨寿旺,李睿,周振达,黄建可. ELISA质控存在的问题探讨[J]. 中国国境卫生检疫杂志, 2011, 0(5): 334-336
作者姓名:杨寿旺  李睿  周振达  黄建可
作者单位:温州国际旅行卫生保健中心;
摘    要:
目的探讨ELISA检测法质量控制方法。方法对目前ELISA质量控制方法及其存在的问题进行详细的分析,寻找其问题存在的原因。结果 ELISA质量控制方法理论在实际应用中存在许多问题,其根源在于作为ELISA质控指标的S/CO值是一比值,不具有溯源性。结论 S/CO值不适宜作为ELISA质量控制指标。

关 键 词:质量控制  酶联免疫吸附试验  样本  吸光度/临界值  质控图  溯源性

The problems in ELISA quality-control
YANG Shou-wang,LI Rui,ZHOU Zhen-da,HUANG Jian-ke Wenzhou International Travel Health Care Center,Wenzhou,Zhejiang ,China. The problems in ELISA quality-control[J]. Chinese Journal of Frontier Health and Quarantine, 2011, 0(5): 334-336
Authors:YANG Shou-wang  LI Rui  ZHOU Zhen-da  HUANG Jian-ke Wenzhou International Travel Health Care Center  Wenzhou  Zhejiang   China
Affiliation:YANG Shou-wang,LI Rui,ZHOU Zhen-da,HUANG Jian-ke Wenzhou International Travel Health Care Center,Wenzhou,Zhejiang 325000,China
Abstract:
Objective To study ELISA quality control method.Methods The current ELISA quality control method and the problems were analyzed and the reasons of the problem existing were found.Results There were many problems in practical application of the current theory of ELISA quality control method,it roots in S/CO as the ELISA quality control index was a ratio value,did not have traceability.Conclusion S/CO values are not appropriate for the index of ELISA quality control.
Keywords:Quality control  ELISA  Sample  Cut-off absorbance ration  Levey-Jenning  Traceability  
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