| 何首乌毛状根培养及大黄酚的含量测定 |
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| 引用本文： | 王振华,杜勤,刘浩,许晓峰,张俊荣,简慧兰,李莉,曾绍培. 何首乌毛状根培养及大黄酚的含量测定[J]. 中草药, 2001, 32(8): 695-696 |
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| 作者姓名： | 王振华 杜勤 刘浩 许晓峰 张俊荣 简慧兰 李莉 曾绍培 |
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| 作者单位： | 广州中医药大学中药学院, |
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| 基金项目： | 本研究为广东省中医药管理局课题B96-31的-部分研究内容 |
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| 摘 要： | 目的 测定何首乌毛状根中大黄酚的含量。方法 用发根农杆菌Ri 15834菌株感染何首乌茎、叶外植体，均可诱导出毛状根，并能合成大黄酚，采用薄层扫描法测定毛状根中大黄酚。结果 毛状根中大黄酚的含量为0．0164％。结论 该毛状根具有典型的毛状根形态特征，大黄酚含量低于何首乌药材。
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| 关 键 词： | 何首乌 毛状根 大黄酚 食量 蒽醌类化合物 卵磷脂 微量元素 |
| 文章编号： | 0253-2670(2001)08-0695-02 |
| 收稿时间： | 2000-09-17 |
| 修稿时间： | 2000-09-17 |
Culturing of hairy root of Polygonum multiforum and determination of its chrysophanol content |
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| WANG Zhenhu,DU Qin,LIU Hao,XU Xiaofeng,ZHANG Junrong,JIAN Huilan,LI Li and ZENG Shaopei. Culturing of hairy root of Polygonum multiforum and determination of its chrysophanol content[J]. Chinese Traditional and Herbal Drugs, 2001, 32(8): 695-696 |
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| Authors: | WANG Zhenhu DU Qin LIU Hao XU Xiaofeng ZHANG Junrong JIAN Huilan LI Li ZENG Shaopei |
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| Affiliation: | College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou Guangdong 510407, China;College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou Guangdong 510407, China;College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou Guangdong 510407, China;College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou Guangdong 510407, China;College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou Guangdong 510407, China;College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou Guangdong 510407, China;College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou Guangdong 510407, China;College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou Guangdong 510407, China |
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| Abstract: | Object To determine the content of chrysophanol in the cultured hairy root of Polygonim multiforum Thunb Methods Hairy root of P multiforum was induced from stem and leaf explant with agrobacterum rhizogens Ri 15834, and its chrysophanol content determined by TLCS Results The transferred root showed typical hairy characteristics and could synthesize chrysophanol to a content of 0 016 4% as determined by TLCS Conclusion Content of chrysophanol in the cultured hairy root was lower than that of the crude medicinal material |
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| Keywords: | Polygonum multiforum Thunb hairy root chrysophanol |
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