SNHG7-miR-653-5p-STAT2反馈通路在神经母细胞瘤进展中的调节作用研究

目的 研究lncRNA SNHG7在神经母细胞瘤(neuroblastoma,NB)进展中的作用,以及SNHG7-miR-653-5p-STAT2反馈通路在神经母细胞瘤进展中的调节作用.方法 从青岛大学附属医院收治的NB患儿体内获得92对NB组织及相邻非肿瘤组织.采用qRT-PCR检测SNHG7在神经母细胞瘤肿瘤组织及...

Role of SNHG7-miR-653-5p-STAT2 feedback loop in regulating neuroblastoma progression

Objective To explore the role of lncRNASNHG7 in the progression of neuroblastoma(NB)and examine the regulatory role of snhg7-mir-653-5p-stat2 feedback pathway in NB.Long noncoding RNAs(lncRNAs)have been implicated in the pathogenesis of various cancers,including neuroblastoma(NB).However,the role of lncRNASNHG7 in NB progression remains elusive.Methods The expression of SNHG7 in NB tissues and cells was detected by quantitative realtime-polymerase chain reaction(qRT-PCR).Cellular invasion and migration were detected by Transwell invasion and migration assay.Luciferase assay was utilized for detecting the interaction between mir-653-5p,SNHG7 and STAT2.The relative expressions of SNHG7 and mir-653-5p in NB were determined by RNA-binding protein immunoprecipitation(RIP)assay and RNA pull-down assay.Spearman's correlation curve was employed for examining the relationship between SNHG7 and mir-653-5p and STAT2.Overall survival was measured by Kaplan-Meier analysis.The effects of SNHG7 on SK-N-SH/SH-SY5Y cells were determined by methylthiazolyldiphenyl-tetrazolium bromide(MTT)and colony formation.Results qRT-PCR demonstrated that,among 92 pairs of neuroblastoma(NB)and adjacent non-tumor tissues,expression quantity of SNHG7 was higher in tumor tissues(n=53)than that in non-tumor tissues(n=39).Kaplan Meier analysis revealed that overall survival decreased with advancing months in high SNHG7 patients(n=53)and similarly in low SNHG7 counterparts(n=39)with a lower extent(P=0.004).An up-regulation of SNHG7 was correlated with a poor overall survival rate of NB patients.Cell function test indicated that a down-regulation of SNHG7 by MTT and colony formation significantly suppressed the survival and proliferation of SK-N-SH/SH-SY5Y cells.Transwell test indicated that knocking down SNHG7 significantly suppressed the migration and invasion of SK-N-SH/SH-SY5Y cells(P<0.01).Luciferase assay revealed that miR-653-5p lowered the luciferase activity of SNHG7-wt(wildtype).And a specific interaction existed between miR-653-5p and STAT2-wt(wildtype).Spearman's correlation curve analysis indicated that SNHG7 in NB tissues was negatively correlated with miR-653-5p(r=-0.281,P=0.007).The expression of STAT2 was negatively correlated with miR-653-5p in NB tissues(r=-0.295,P=0.004)and positively correlated with SNHG7 in NB tissues(r=0.296,P=0.004).These data suggested that SNHG7 played a role in NB progression by regulating the pathway of miR-653-5p/STAT2.Conclusion SNHG7 promotes NB progression through the pathway of miR-653-5p/STAT2.Thus it may provide a novel therapeutic target and prognostic biomarker for NB.