神经生长因子在周围神经侧枝发芽中的表达

目的：研究周围神经端侧吻合后侧支发芽过程中神经生长因子（NGF）的表达,探讨端侧吻合后神经侧支发芽再生的机制.方法：SD大鼠共32只,建立胫腓神经端侧吻合模型,随机分为1周、2周、4周和8周组,切取吻合近端和远端神经纵切片标本,进行神经生长因子的免疫组织化学反应,并结合计算机图像分析进行反应强度的半定量分析.结果：端侧吻合后近端神经NGF表达未见显著增强,而在远端的表达则显著增强.结论：端侧吻合远侧端NGF在神经侧支发芽中起着十分重要的作用.

The expression of nerve growth factor in lateral sprouting after peripheral nerve end-to-side anastomosis

Objective To explore the expression of nerve growth factor in lateral sprouting after peripheral nerve end-to-side anastomosis and find the mechanism of lateral sprouting. Methods 32 SD rats were randomly divided into four groups: 1 week group, 2 week group, 4 week group and 8 week group. Immunohistochemical analysis by the computer image analysis was performed to study the expression of the NGF after end-to-side anastomosis. Results Increased expression of NGF were observed in distal ends after end-to-side anastomosis, and on more expression of NGF in proximal ends was also observed. Conclusion It show that the nerve growth factors in distal ends may be acts as an important role in lateral sprouting.