内皮型一氧化氮合酶基因G894T多态性与心肌梗死的相关性

目的探讨内皮型一氧化氮合酶(eNOS)基因G894T多态性与心肌梗死的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析,聚丙稀酰胺凝胶电泳方法检测确诊的225例冠心病患者和171名健康人的内皮型eNOS基因G894T多态性。结果①病例组与对照组比较GT TT基因型频率较高(65/225和27/171),P<0.01,病例组T等位基因频率高于对照组(0.14和0.079)。②心肌梗死组GT TT基因型频率高于非心肌梗死组(34/108和18/117),P<0.01,T等位基因频率高于非心肌梗死组(0.16和0.08),P<0.01。结论eNOS基因GT TT基因型冠心病频率较高,并且易发生心肌梗死。

The correlation between endothelial nitric oxide synthase gene G894T polymorphysim and myocardial infarction

Objective To investigate the distribution of the G894T polymorphysim at exon 7 in the endothelial nitric oxide synthase gene in Chinese Han nationality and study the correlation between the G894T mutation at exon 7 in the endothelial nitric oxide synthase gene with myocardial infarction (MI). Methods G894T polymorphysim at exon 7 in the endothelial nitric oxide synthase gene was measured in 225 patients with coronary heart disease(CHD) and 171 unrelated health individuals in control by a combination of polymerase chain reaction-restriction fragment length polymorphysm(PCR-RFLP) and direct AgNO3 staining technique. Results The frequencies of the GG GT genotypes were higher in patients with CHD(P<0.01). The GG GT genotypes were associated with the history of myocardial infarction(P<0.01).Conclusion The results indicated that the G894T polymorphysim at exon 7 in the endothelial nitric oxide synthase gene in Chinese Han nationality was associated with coronary heart disease. The individual with GT or TT genotype is susceptible to myocardial infarction.