新疆伊犁地区马和驴博尔纳病病毒自然感染的调查

目的 调查新疆伊犁地区伊犁马和伊犁驴博尔纳病病毒(Boma disease virus,BDV)流行现状,分析BDV种系来源.方法 采用荧光定量巢式实时逆转录聚合酶链反应(fluorescence quan-titative nested RT-PCR,FQ-nRT-PCR),对新疆伊犁地区518匹伊犁马和206头伊犁驴外周血单个核细胞 (peripheral blood mononuclear cell,PBMC)进行BDV p24基因片段检测.对榆测阳性结果 的标本通过BDV p40和质粒标准品的检测进行进一步验证并测序,对其进行基因同源性、氨基酸序列和系统发生分析.结果 5例伊犁马和4例伊犁驴血标本BDV p24榆测阳性,阳性率分别为0.97%和1.94%;9例标本BDV tn0片段检测均阳性,质粒标准品榆测均阴性;BDV p24扩增产物序列与He/80株的同源性为100%.结论 新疆伊犁地区伊犁马和伊犁驴中可能存在BDV的自然感染,该地区BDV流行株与He/80株存在同源性.

Epidemiological investigation of Borna disease virus infection in horses and donkeys in Yili, Xinjiang

Objective To investigate the epidemiology of BDV infection in Yili horses and Yili donkeys and to analyze phylogenetic source of BDV in Yili area, Xinjiang. Methods We established fluo- rescence quantitative nested RT-PCR to detect BDV p24 segment in peripheral blood mononuclear cells (PBMCs) of 518 Yili horses and 206 Yili donkeys in Yili area, Xinjiang. Positive products were validated by detecting BDV p40 segment and plasmid to preclude the contamination, and were sequenced to analyze the homology of gene sequence, amino acid sequence and phylogenetic tree. Results The positive rates of BDV infection in PBMCs of 518 Yili horses and 206 Yili donkeys were 0.97% and 1.94%, respectively. The results of BDV p40 segment verification were positive in all of the samples of BDV p24 positive. All the samples tested were not contaminated by plasmid. There was a homology of the gene sequence of positive PCR samples with strain He/80. And the gene sequence revealed more than 93% identical to H1766 and strain V. Conclusion Our study suggested BDV natural infection in Yili horses and Yili donkeys. The en- demic BDV had a high degree of identity to strain He/80.