重度联合免疫缺陷小鼠的繁育及其应用初报

应用带过滤罩的透明鼠在层流柜内繁育Ｔ和Ｂ淋巴细胞缺失的重度联合免疫缺陷（ｓｃｉｄ）小鼠获得成功，１９９３年５月至１９９４年４月，共繁育ｓｃｉｄ小鼠２２１只，寿命均超过１２个月。平均每窝产仔数为５．５只，显著低于对照组ＢＡＬＢ／ｃ小鼠，不育率为１４．９％，离乳率为８８．２％，与ＢＡＬＢ／ｃ小鼠相比均无明显差异。ｓｃｉｄ小鼠４～１０周龄的体重与同龄ＢＡＬＢ／ｃ小鼠相比无明显差异，微生物学检测均符合ＳＰ

Preliminary Report on the Breeding and Rearing of SCID Mice and Its Application

Severe combined immunodeficiency (SCID) mice, deficient for both T and Blymphocytes, had been bred successfully in laminar air flow units. The life sPansof most SCID mice were over 12 months, the average litter size 5 .5, the reproductiveand weaning rate were comparable to those of BALB/c mice. The results ofmicrobiological examination showed that the animals were free from the specificpathogens. It was also revealed that the thymus and spleen were significantly smallerthan those of BALB/c mice, circulating lymphocytes comprised only a small fraction of the total leukocytes in the peripheral blood (11%-26% ), while neutrophilsamounted to 74%-87%. Three kinds of human tumor tissues (lung carcinoma.liver cell carcinoma and mucinous carcinoma of colon) xenografted in SCID micegrew much more faster than those in BALB/c nude mice.