基于Notch信号通路探讨针刺曲池、足三里对MCAO大鼠的神经保护机制

目的:观察针刺曲池穴及足三里穴对MCAO大鼠的行为学干预效应,同时评价其对Notch信号通路标志性蛋白的影响。方法:将36只SD大鼠随机分成空白组、模型组及针刺组,各12只。模型组及针刺组大鼠均接受改良线栓法建立左侧大脑中动脉局灶性缺血(MCAO)再灌注模型,空白组大鼠仅进行血管分离术,术后空白组及模型组大鼠每日接受模拟捉拿1次,针刺组大鼠予针刺曲池穴及足三里穴,连续干预14 d,用神经行为学评分评估大鼠行动能力,TTC染色法观察脑梗死体积变化,HE染色法观察大鼠脑组织细胞形态和结构,Western blotting法检测各组大鼠脑组织Notch1、Hes1蛋白表达变化。结果:1)与模型组比较,针刺组大鼠神经行为学评分明显提高,并缩小了脑梗死体积;2)针刺可明显改善MCAO术大鼠脑组织细胞形态和结构;3)Western blot结果显示针刺可明显上调大鼠脑组织Notch1、Hes1的蛋白水平。结论:电针曲池穴及足三里穴可改善MCAO大鼠行为能力,其作用机制可能与介导Notch通路有关。

Mechanism for Neuroprotection against MCAO Rats of Acupuncturing at Quchi (LI 11) and Zusanli (ST 36) based on Notch Signaling Pathway

To observe the behavioral effects of acupuncture at Quchi (LI 11) and Zusanli (ST 36) acupoints on MCAO rats and to evaluate the effects on Notch signaling pathway marker proteins. Methods:A total of 36 SD rats were randomly divided into blank group, model group and acupuncture group, with 12 rats in each group. The rats in the model group and the acupuncture group received the modified suture method to establish the model of left middle cerebral artery occlusion (MCAO) reperfusion. The rats in the blank group were only subjected to vascular segmental surgery. The postoperative blank control group and model group rats were subjected to simulated catching once a day. Rats in acupuncture group were given acupuncture at Quchi and Zusanli points for continuous intervention for 14 days. Neurobehavioral scores were used to assess the ability of rats to exercise. TTC staining was used to observe changes of cerebral infarction volume. HE staining was used to observe morphology and structure of rat brain cells. The expression of Notch1 and Hes1 protein in brain tissue of each group was detected by Western blotting. Results:1) Compared with the model group, neurobehavioral score of acupuncture group was significantly increased and the volume of cerebral infarction was reduced; 2) Acupuncture significantly improved the morphology and structure of brain cells in MCAO rats; 3) Western blotting results showed that acupuncture significantly increased the protein levels of Notch1 and Hes1 in rat brain. Conclusion:Electroacupuncture at Quchi and Zusanli points can improve the behavioral abilities of MCAO rats, which may be related to the mechanism of Notch pathway.