雷公藤属植物叶片中萜类成分的差异性

目的:测定雷公藤属植物叶片中5种萜类成分的含量并比较差异,研究3个物种间的化学亲缘关系。方法:采用HPLC法测定28批雷公藤属植物叶片中雷公藤甲素、雷公藤内酯酮、雷酚新内酯、雷酚内酯和雷公藤红素5个成分的含量,并进行方差分析和聚类分析。结果:雷公藤属植物叶片中,雷公藤甲素、雷酚内酯和雷公藤红素的含量差异有统计学意义(P0.05);雷公藤内酯酮和雷酚新内酯含量差异无统计学意义(P0.05)。运用系统聚类将28批雷公藤属植物聚为4类:第I类为东北雷公藤和昆明山海棠;第II类为雷公藤;第III类为雷公藤;第IV类为雷公藤和昆明山海棠。结论:雷公藤属植物叶片中部分萜类成分含量差异明显。东北雷公藤与雷公藤化学亲缘关系较远;昆明山海棠混在2者之间,与2者无明显区分。

Study on Differences of Terpenoid Compositions in Plant Leaves of Tripterygium Genus

To study the relative relationship among the three species of Tripterygium genus by measuring the contents of 5 terpenoid compositions in plant leaves of Tripterygium genus and comparing the differences.Methods:HPLC was used to measure the 5 composition contents including Triptolide,Triptonide,Neotriptophenolide,Triptophenolide and Tripterine in 28 batches of plant leaves of Triptergium genus.ANOVA and cluster analysis were also performed to verify the results.Results:There were significant differences among the contents of Triptolide,Triptophenolide and Tripterine in the plant leaves of Triptergium genus (P<0.05); There was no significant difference between Triptonide and Neotriptophenolide.Hierarchical clustering was used to divide the 28 batches of plants of Triptergium genus into 4 kinds:I:Tripterygium regelii Sprague et Takeda and Tripterygium hypoglaucum; II:Tripterygium; III:Tripterygium; IV:Tripterygium and Tripterygium hypoglaucum.Conclusion:The differences of some contents of terpenoid compositions in plant leaves of Tripterygium genus were significant.The relative relationship of Tripterygium regelii Sprague et Takeda and Tripterygium was distant; Tripterygium hypoglaucum was between the two,which had no significant difference.