炮制对决明子中六种游离蒽醌含量的影响

目的:比较炮制对决明子中主要活性成分游离蒽醌含量的影响。方法:采用高效液相色谱法检测决明子中6种游离蒽醌类成分的含量,分别为橙黄决明素,大黄酸,决明蒽醌,大黄素,大黄酚,大黄素甲醚,并比较其炮制前后的含量变化。结果:炮制后6种游离蒽醌变化情况为,橙黄决明素和大黄酸的含量变化没有显著性差异,决明蒽醌的含量升高了25%,大黄素的含量升高了21%,大黄酚的含量降低了15%,大黄素甲醚的含量降低了21%。结论:炮制对决明子中主要活性成分游离蒽醌类成分的含量会产生不同的影响,有些游离蒽醌含量保持不变,有些游离蒽醌含量会有显著升高,有些游离蒽醌含量会明显降低。

Effects of Processing on the Content of Six Free Anthraquinones in Semen Cassiae

To compare the effects of processing on free anthraquinones which were the main active ingredient in Semen Cassiae. Methods:The content of six free anthraquinones in Semen Cassiae was determined by the high-performance liquid chromatographic method and compares the content changes before and after processing. The six free anthraquinones were aurantio-obtusin, rhein, obtusifolin, emodin, chrysophanol and physcion. Results There was no significant difference in the content changes of aurantio-obtusin and rhein, and the content of obtusifolin was increased by 25%. The content of emodin was increased by 21%, and the content of chrysophanol was decreased by 15%. The content of physcion was decreased by 21% after processing. Conclusion:Processing has different effects on the content of free anthraquinones. Some free anthraquinones contents remained unchanged while some increased significantly, and some decreased significantly.