| 血清饥饿法对人牙髓细胞周期同步化的研究 |
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| 引用本文： | 刘斐,吴补领,高杰,黄欣,麻丹丹,陈婷. 血清饥饿法对人牙髓细胞周期同步化的研究[J]. 牙体牙髓牙周病学杂志, 2011, 0(2): 67-71 |
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| 作者姓名： | 刘斐 吴补领 高杰 黄欣 麻丹丹 陈婷 |
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| 作者单位： | 南方医科大学南方医院口腔科;南方医科大学口腔医学院; |
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| 基金项目： | 教育部博士点新教师基金项目(20094433120004); 广东省科技计划项目(2008B011300018-8) |
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| 摘 要： | 目的:探讨不同的血清饥饿方法和饥饿时间对人牙髓细胞(HDPCs)细胞周期的影响.方法:血清直接饥饿和梯度饥饿处理人牙髓细胞.倒置显微镜观察细胞形态;CCK-8检测血清饥饿处理对HDPCs增殖能力的影响;流式细胞术检测细胞周期验证HDPCs同步化的效果.结果:血清直接饥饿处理或梯度饥饿处理后其G0/G1期HDPCs的比例...
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| 关 键 词： | 血清饥饿法 人牙髓细胞 细胞周期同步化 G0/G1期 |
Effects of serum starvation on cell cycle synchronization in human dental pulp cells |
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| LIU Fei,WU Bu-ling,GAO Jie,HUANG Xin,MA D,an,CHEN Ting. Effects of serum starvation on cell cycle synchronization in human dental pulp cells[J]. Chinese Journal of Conservative Dentistry, 2011, 0(2): 67-71 |
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| Authors: | LIU Fei WU Bu-ling GAO Jie HUANG Xin MA D an CHEN Ting |
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| Affiliation: | LIU Fei,WU Bu-ling,GAO Jie,HUANG Xin,MA Dandan,CHEN Ting(Department of Stomatology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China) |
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| Abstract: | AIM: To examine the effects of serum starvation on cell cycle synchronization of human dental pulp cells(HDPCs) by treating HDPCs with different starvation methods and starvation times.METHODS: HDPCs were treated with serum direct starvation and gradient starvation.The morphological changes of HDPCs were observed by an inverted microscope.CCK-8 assay was used to investigate the effects of serum starvation on cell proliferation,and cell cycle distribution of HDPCs was detected by flow cytometry(FCM).RESULTS:... |
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| Keywords: | serum starvation HDPCs synchronization of the cell cycle G0/G1 stage |
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