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Antibody response in laying hens with small amounts of antigen
Authors:Anders Larsson  David Carlander  Martin Wilhelmsson
Affiliation:1. Department of Clinical Chemistry , University Hospital , Uppsala , S‐75185 , Sweden Phone: 46 18 663000 Fax: 46 18 663000 E-mail: anders.larsson@clinchem.uas.se;2. Department of Clinical Chemistry , University Hospital , Uppsala , S‐75185 , Sweden;3. Department of Animal Breeding and Genetics , Swedish University of Agricultural Sciences , Uppsala , Sweden
Abstract:
Chicken antibodies offer many advantages over the traditional mammalian ones. A laying hen produces large amounts of yolk antibodies and the use of yolk antibodies eliminates the painful procedure of collecting blood from the animal. Thus, the use of chicken antibodies will reduce both the number of animals required to produce antibodies and also animal distress. Chicken antibodies also have several biochemical advantages compared to mammalian antibodies: they often increase the signal and reduce interference in many assays. However, the species chosen for antibody production have usually been mammals. This is probably due to tradition, but also to limited knowledge about the production of chicken antibodies. We studied the immune response in the chicken using small amounts of mammalian antigen, and show that a good immune response can be obtained with 0.1–1.0 μg of bovine serum albumin.
Keywords:Antibody  chicken  egg‐yolk  ELISA  immunization  plasma
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