川芎嗪抑制大鼠气道平滑肌细胞增殖的作用

目的探讨川芎嗪对大鼠气道平滑肌细胞(airway smooth muscle cells,ASMCs)增殖的影响。方法采用酶消化法和改良组织块法培养原代大鼠气道平滑肌细胞。MTT法检测血小板源生长因子(platelet-derived growth factor,PDGF)与川芎嗪共同处理的大鼠ASMCsA490的吸光度值,以观察川芎嗪对PDGF诱导的细胞增殖的抑制作用。Western blot检测ERK1/2及p-ERK1/2蛋白表达水平。结果 MTT法检测,与各对照组比较,给予12.5、25、50、100和200μmol.L-1浓度川芎嗪处理6、12、24、36和48h后,各浓度组川芎嗪处理的细胞平均抑制率均增加,P<0.05,其中以200μmol.L-1在48h时效果最明显,P<0.01。川芎嗪(200μmol.L-1)与PDGF(20pg·L-1)共同处理30min和60min后,p-ERK1/2蛋白表达水平均明显降低。结论川芎嗪对增殖的ASMCs有抑制作用,可能与抑制ERK1/2的信号通路活化有关。

Inhibition of Tetramethylpyrazine on the proliferation f rat airway smooth muscle cells

Aim To investigate the effect of Tetramethylpyrazine (TMP) on the proliferation of airway smooth muscle cells (ASMCs). Methods Primary ASMCs of rats were cultured. The absorbance (A490) value of ASMCs treatment with platelet-derived growth factor (PDGF) in the presence of TMP was detected by MTTto observe the anti-proliferation of TMP. The levels of ERK1/2 and p-ERK1/2 proteins were determined by Western blot.Results In presence of the TMP with different concentrations (12.5,25,50,100 and 200 μmol·L-1) at 6,12,24,36 and 48 hours,compared with control groups,the average inhibitory rates of cell proliferation in all groups were increased significantly (P<0.05),especially of 200μmol·L-1 TMP group at 48 hours (P<0.01). The levels of p-ERK1/2 proteins were significantly decreased at 30 minutes and 60 minutes after treatment with TMP (200μmol·L-1) and PDGF (20 pg·L-1). Conclusion The proliferation of ASMCs is inhibited by TMP,and this may be related to inhibiting the activation of ERK1/2 signaling pathway.