| HPLC法测定烫伤油及烫伤膜中大黄素的含量 |
| |
| 引用本文： | 李嵘,吴健,谭家风,徐康康,李燕. HPLC法测定烫伤油及烫伤膜中大黄素的含量[J]. 南京中医药大学学报, 2002, 18(5): 300-301 |
| |
| 作者姓名： | 李嵘 吴健 谭家风 徐康康 李燕 |
| |
| 作者单位： | 南京医科大学附属南京儿童医院,江苏,南京,210008;南京农业大学,江苏,南京,210029 |
| |
| 摘 要： | 目的 建立烫伤膜及烫伤油中大黄素的含量测定方法,方法 采用HPLC法,C18柱,流动相为甲醇-1%磷酸水溶液,检测波长为254nm。结果 大黄素在7.20min出峰,烫伤膜,烫伤油的平均回收率分别为96.37%和97.9%。RSD分别为0.64%和1.11%。结论 烫伤油改为膜剂后,其主要成分大黄素的含量高于原有油剂,此检测方法简单快速,准确实用。
|
| 关 键 词： | 烫伤膜 烫伤油 大黄素 高效液相色谱法 含量测定 |
| 文章编号： | 1000-5005(2002)05-0300-02 |
| 修稿时间： | 2002-05-05 |
HPLC Determination of Content of Emodin in Scald Oil and Scald Membrane |
| |
| LI Rong ,WU Jian ,TAN Jia feng ,XU Kang kang ,LI Yan. HPLC Determination of Content of Emodin in Scald Oil and Scald Membrane[J]. Journal of Nanjing University of Traditional Chinese Medicine(Natural Science), 2002, 18(5): 300-301 |
| |
| Authors: | LI Rong WU Jian TAN Jia feng XU Kang kang LI Yan |
| |
| Affiliation: | LI Rong 1,WU Jian 2,TAN Jia feng 1,XU Kang kang 1,LI Yan 1 |
| |
| Abstract: | OBJECTIVE To establish the method for the determination of the content of emodin in Scald Oil and Scald Membrane.METHOD HPLC method was used, with C 18 column, methanol-phosphoric acid water solution as the mobile phase and 254 nm wavelength.RESULT Emodin reached its peat at 7.2 min, the recovery rates of Scald Membrane and Scald Oil were 96.37% and 97.9% respectively and RSD were 0.64% and 1.11% respectively. CONCLUSION After Scald Oil changes into membrane form, the content of the main component emodin is higher than the oil form. This method of determination is quick, accurate and practical. |
| |
| Keywords: | Scald Membrane Scald Oil Emodin HPLC content determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
