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| 上调miRNA-145表达对非小细胞肺癌细胞增殖、凋亡及放射敏感性的影响 | |
| 引用本文： | 王亚飞,宋长亮,张振军,杨琼,杨庚武,张磊,田云霄,宋小天. 上调miRNA-145表达对非小细胞肺癌细胞增殖、凋亡及放射敏感性的影响[J]. 癌症进展, 2019, 17(10): 1152-1155 |
| 作者姓名： | 王亚飞 宋长亮 张振军 杨琼 杨庚武 张磊 田云霄 宋小天 |
| 作者单位： | 邯郸市中心医院肿瘤科,河北 邯郸,056000;邯郸市骨科医院骨科,河北 邯郸,056000;邯郸市中心医院病理科,河北 邯郸,056000;河北医科大学基础医学院免疫学教研室,石家庄,0500170 |
| 摘 要： | 目的探讨上调miRNA-145表达对非小细胞肺癌细胞增殖、凋亡及放射敏感性的影响。方法收集人正常肺上皮细胞株BEAS-2B和非小细胞肺癌细胞株A549,采用逆转录聚合酶链反应(RT-PCR)检测两种细胞中miRNA-145的相对表达量。以Lipofectamine^TM 2000转染试剂将miRNA-145模拟物和阴性对照质粒转染至A549细胞中,分别作为miRNA-145组和NC组,以只加入转染试剂的A549细胞作为对照组,RT-PCR检测各组细胞中miRNA-145的相对表达量。采用四甲基偶氮唑蓝(MTT)法和流式细胞术(FCM)检测上调miRNA-145表达对细胞增殖和凋亡的影响。给予不同放射剂量X线照射后采用克隆形成实验检测细胞的放射敏感性。结果A549细胞中miRNA-145的相对表达量明显低于正常肺上皮BEAS-2B细胞(P<0.01);转染后,miRNA-145组细胞中miRNA-145的相对表达量高于对照组(P<0.05);转染后,NC组与对照组细胞中miRNA-145的相对表达量比较,差异无统计学意义(P﹥0.05);miRNA-145组细胞转染24、48、72 h的光密度(OD)值均低于对照组(P<0.05);转染48 h后,miRNA-145组细胞的凋亡率高于对照组(P<0.05);2、4、6、8 Gy剂量的X线照射后miRNA-145组细胞的存活分数(SF)均低于对照组,放射增敏比(SER)为1.491。结论与正常肺上皮BEAS-2B细胞比较,A549细胞中miRNA-145的相对表达量较低,上调其表达能够抑制A549细胞增殖,促进细胞凋亡,增强放射敏感性。 |
| 关 键 词： | 非小细胞肺癌 miRNA-145 细胞增殖 细胞凋亡 放射敏感性 |
Effect of up regulation of miRNA-145 on cell proliferation,apoptosis and radiosensitivity in non-small cell lung cancer |
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| WANG Yafei,SONG Changliang,ZHANG Zhenjun,YANG Qiong,YANG Gengwu,ZHANG Lei,TIAN Yunxiao,SONG Xiaotian. Effect of up regulation of miRNA-145 on cell proliferation,apoptosis and radiosensitivity in non-small cell lung cancer[J]. Oncology Progress, 2019, 17(10): 1152-1155 | |
| Authors: | WANG Yafei SONG Changliang ZHANG Zhenjun YANG Qiong YANG Gengwu ZHANG Lei TIAN Yunxiao SONG Xiaotian |
| Affiliation: | (Department of Oncology,Handan Central Hospital,Handan 056000,Hebei,China;Department of Orthopedics,Orthopedics Hospital of Handan City,Handan 056000,Hebei,China;Department of Pathology,Handan Central Hospital,Handan 056000,Hebei,China;Department of Immunology,Basic Medical Sciences of Hebei Medical University,Shijiazhuang 050017,Hebei,China) |
| Abstract: | WANG Yafei;SONG Changliang;ZHANG Zhenjun;YANG Qiong;YANG Gengwu;ZHANG Lei;TIAN Yunxiao;SONG Xiaotian(Department of Oncology,Handan Central Hospital,Handan 056000,Hebei,China;Department of Orthopedics,Orthopedics Hospital of Handan City,Handan 056000,Hebei,China;Department of Pathology,Handan Central Hospital,Handan 056000,Hebei,China;Department of Immunology,Basic Medical Sciences of Hebei Medical University,Shijiazhuang 050017,Hebei,China) |
| Keywords: | non-small cell lung cancer miRNA-145 cell proliferation cell apoptosis radiosensitivity |
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