山葡萄多酚对大鼠心肌线粒体氧化损伤的保护作用

目的:在大鼠心肌线粒体（Mt）自由基损伤的体外实验中观察山葡萄多酚(PVAR)对心肌线粒体氧化损伤的保护作用，为治疗心肌缺血再灌注损伤提供实验依据。方法: 实验分5组，正常线粒体组、诱导损伤线粒体组和预先加25、50、100 mg•L^-1PVAR再诱导损伤线粒体组；利用Fe²⁺/维生素C（VitC）系统产生氧自由基，体外对Wistar大鼠心肌Mt诱导损伤，观察各组Mt心磷脂和MDA含量、膜流动性、ATPase活性和肿胀度的变化。结果：与损伤组比较，三种剂量PVAR组心磷脂含量明显增高（P<0.05)，MDA明显减少（P<0.01)，膜流动性显著增大(P<0.01)，ATPase活性增高（P<0.05)，100 mg•L^-1PVAR组线粒体肿胀度与正常组比较差异无显著性。结论:氧自由基引起Mt损伤；PVAR对氧自由基引起的大鼠心肌Mt损伤具有保护作用，保护机制可能是直接减少氧自由基的生成。

Effects of polyphenols of vitis amurensis Rupr on rat heart mitochondria injury induced by vitamin C and FeSO4

Objective To study the protective effects of the polyphenols of vitis amurensis Rupr(PVAR) on rat heart mitochondria injury induced by oxygen radicals.Methods Experiment was designed into 5 groups:normal group,injury group and 25,50,100 mg·L-1PVAR+injury group.In experiment of rat heart mitochondria injury in vitro,vitamin C and FeSO4 played as an injury agent,PVAR played as a protective agent.The cardiolipin and MDA level of the mitochondria were determined.The membrane fluidity,ATPase activity and swelling of the mitochondria were examined.Results Compared with injury group,the cardiolipin was increased(P<0.05),the MDA was decreased(P<0.05),the membrane fluidity and the ATPase activitiy were increased(P<0.01) in 25,50,100 mg·L-1 PVAR groups.There was no significant difference of swelling of injured mitochondria between PVAR( 100 mg·L-1) group and control group.Conclusion Oxygen radicals can result in severe mitochondria injuried.The PVAR can protect the injuried rat heart mitochondria by antagonizing the free radicals.