旋转磁场联合5-Fu对SP2/0细胞周期及凋亡的影响

目的 研究旋转磁场 (RMF) 联合氟尿嘧啶（5-Fu）对小鼠骨髓瘤细胞 (SP2/0) 细胞周期及凋亡的影响。方法 SP2/0细胞分为四组：对照组、单纯5-Fu化疗组（单化组）、单纯磁疗组（单磁组）、磁场联合5-Fu组（磁化组）。用RMF联合5-Fu作用于SP2/0细胞，流式细胞仪进行分析。结果 单磁组S期细胞比例和单化组G1期细胞比例均高于其余3组（P<0.05）。磁化组S期细胞比例降低，低于单磁组，但仍高于对照组和单化组（P<0.05）。磁化组G1期比例高于对照组及单磁组（P<0.05）。单磁组和对照组比较，细胞凋亡率差异无统计学意义（P>0.05）；单化组和磁化组的细胞凋亡率明显高于对照组和单磁组（P<0.05），而磁化组的凋亡率明显高于单化组（P<0.05）。结论 磁场可引起细胞周期S期比例增高。单纯磁场处理不能诱发细胞的凋亡，但磁场可增强5 Fu的毒性从而促进5 Fu引起的细胞凋亡，其机制可能与RMF引起SP2/0细胞周期的改变有关。

Effect of Rotary Magnetic Field Combining 5-fluorouracil on Cell Cycle and Apoptosis in SP2/0 Cell Line

Abstract: Objective To study the effect of rotary magnetic field (RMF) combining 5-Fu on the cycle and apoptosis of mouse cell line SP2/0 in vitro.Methods SP2/0 cells were randomly divided into four groups: control group (N), 5-Fu group (C), magnetic group (M) and magnetic combining 5-Fu group (M+C).The M and M+C groups were treated with a RMF for two hours once a day.On day 4, the C and M+C groups were treated with 5-Fu 20 μg/ml.On day 5, cell cycle and apoptosis were measured by the flow cytometric (FCM).Results The S phase proportion of the M group and the G1 phase proportion of the C group were higher than that of the other three groups（P<0.05）.The S phase proportion of the M+C group decreased and lower than that of the M group，but was still higher than that of the N and C groups（P<0.05）.There was no significant difference in apoptosis rates between the N and M groups（P>0.05）.The apoptosis rates of the C and M+C groups were remarkedly higher than those of the N and M groups and the M+C group had the highest apoptosis rate.Conclusion The RMF cant induce the apoptosis.But it can enhance the cytotoxicity of 5-Fu and promote the cell apoptosis.The mechanism of the apoptosis may be related to SP2/0 cell line arrested at S phase.