| 无毒棉籽水提物中抗抑郁活性成分的筛选研究 |
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| 引用本文： | 李云峰,刘艳芹,杨明,王恒林,赵毅民,罗质璞.无毒棉籽水提物中抗抑郁活性成分的筛选研究[J].军事医学科学院院刊,2004,28(3):250-251,263. |
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| 作者姓名： | 李云峰 刘艳芹 杨明 王恒林 赵毅民 罗质璞 |
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| 作者单位： | 1. 军事医学科学院毒物药物研究所,北京,100850
2. 解放军第309医院麻醉科,北京,100091 |
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| 基金项目： | 北京市自然科学基金资助项目 ( 70 42 0 5 2 ) |
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| 摘 要： | 目的：筛选无毒棉籽水提物有效部位(CTN)中抗抑郁活性成分。方法：提取大脑皮质突触膜，以CTN或其中5个黄酮类单体成分(分别为CTN-4，CTN-7，CTN-8，CTN-9和CTN-10)与突触膜共孵，放射免疫法测定腺苷酸环化酶(adenylate cyclase．AC)活性。结果：与阳性药丁螺环酮(5-HT1A受体部分激动剂)类似，CTN及CTN-4，7，8，9，10均可剂量依赖性激活突触膜AC，其中CTN-8在5个黄酮类单体中活性最强。结论：黄酮类化合物槲皮素-3-O-芹菜糖基芦丁糖苷(即CTN-8)可能是CTN中最强的抗抑郁活性成分之一，并且可能是5-HT1A受体激动剂。
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| 关 键 词： | 棉籽 腺苷酸环化酶 抗抑郁剂 药物筛选 |
| 文章编号： | 1000-5501(2004)03-0250-03 |
Screening of the antidepressant components in aqueous extract of detoxified cottonseeds |
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| LI Yun-Feng ,LIU Yan-Qin ,YANG Ming ,WANG Heng-Lin ,ZHAO Yi-Min ,LUO Zhi-Pu.Screening of the antidepressant components in aqueous extract of detoxified cottonseeds[J].Bulletin of the Academy of Military Medical Sciences,2004,28(3):250-251,263. |
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| Authors: | LI Yun-Feng LIU Yan-Qin YANG Ming WANG Heng-Lin ZHAO Yi-Min LUO Zhi-Pu |
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| Institution: | LI Yun-Feng 1,LIU Yan-Qin 1,YANG Ming 1,WANG Heng-Lin 2,ZHAO Yi-Min 1,LUO Zhi-Pu 1 |
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| Abstract: | Objective: To screen the antidepressant components in aqueous extract of detoxified cottonseeds (CTN). Methods: The synaptic membrane of rat cerebral cortex was extracted and incubated with CTN or its five flavonoid compounds (CTN-4, 7, 8, 9, 10) respectively. The adenylyl cyclase (AC) activity was measured by radioimmunoassay. Results: Just like the positive drug buspirone, a 5-HT 1A receptor partial agonist, CTN and its five flavonoid compounds all activated AC in synaptic membrane in a dose-dependent manner, with compound CTN-8 being the most effective. Conclusion: The CTN-8, quercetin-3-O-apionyl- rutinoside, may be one of the most effective antidepressant components in CTN, and be a 5-HT 1A receptor agonist. |
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| Keywords: | cottonseed adenylate cyclase antidepressants drug screening |
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