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Evaluation of the BD Phoenix Automated Microbiology System SMIC/ID panel for identification and antimicrobial susceptibility testing of Streptococcus spp
Authors:Hirakata Yoichi  Matsuda Junichi  Nakano Michiko  Hayashi Tomomi  Tozaka Shusaku  Takezawa Toshiyuki  Takahashi Hiroshi  Higashiyama Yasuhito  Miyazaki Yoshitsugu  Kamihira Shimeru  Kohno Shigeru
Affiliation:Department of Laboratory Medicine, Nagasaki University School of Medicine and Dentistry, Japan. hirakata@net.nagasaki-u.ac.jp
Abstract:
The BD Phoenix Automated Microbiology System SMIC/ID panel was evaluated for identification and antimicrobial susceptibility testing (AST) of various streptococci. A group of 97 consecutive clinical isolates of Streptococcus pneumoniae, 23 Streptococcus pyogenes, 24 Streptococcus agalactiae, and 34 viridans streptococci were collected and comparisons made with routine manual methods used in the clinical microbiology laboratory. Overall, in 162 (91%) of 178 isolates, Phoenix identification results demonstrated agreement. For AST results for the 162 isolates that demonstrated identification concordance, the overall essential agreement rate was 98.5%; the category agreement was 94.9%; and the very major error, major error, and minor error rates were 0%, 0.15%, and 5.8%, respectively. Although relatively high minor error rates were observed with S. pneumoniae and beta-lactams, 79.2% of the 77 minor errors were the result of a single log(2) dilution difference. The Phoenix SMIC/ID panel performed favorably and demonstrated the advantages of automation and simple methodology.
Keywords:
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