Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells |
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| Authors: | C Czerkinsky G Andersson H P Ekre L A Nilsson L Klareskog O Ouchterlony |
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| Affiliation: | Department of Oral Microbiology, University of G?teborg, Sweden. |
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| Abstract: | ![]()
A reverse modification of ELISPOT assay using nitrocellulose membranes and epitope-specific monoclonal antibodies is described for the detection of single lymphokine-secreting cells. As a model, the production of gamma-interferon by mitogen stimulated human peripheral blood lymphocytes has been examined. The assay can also be modified to permit microscopic examination of spot-forming cells. |
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