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| PCR法检测转基因大豆毛状根的hbFGF基因 | |
| 引用本文： | 马冬雪,董国刚,于金兰,惠金玲,HUI Jin-ling. PCR法检测转基因大豆毛状根的hbFGF基因[J]. 解剖科学进展, 2009, 15(3): 276-278 |
| 作者姓名： | 马冬雪 董国刚 于金兰 惠金玲 HUI Jin-ling |
| 作者单位： | 1. 空军总医院药学部,北京,100142 2. 南空机关医院,南京,210018 3. 空军466医院,北京,100089 |
| 摘 要： | 目的通过检测转基因大豆毛状根的人碱性成纤维细胞生长因子(hbFGF),为利用毛状根生产药物实现工业化提供实验基础。方法采用PCR检测方法,利用植物基因组DNA提取试剂盒,兔抗人bFGF单克隆抗体进行对转基因大豆毛状根hbFGF检测。结果用PCR检测转基因大豆毛根,并在60条Hyg抗性大豆毛状根中鉴定出19条大豆毛状根整合状hbFGF,阳性率为32%。兔抗人bFGF单克隆抗体检测表明,hbFGF在转基因大豆毛状根中稳定表达。结论转基因大豆毛状根能够稳定表达hbFGF基因,在工业生产中具有广阔的前景。 |
| 关 键 词： | 转基因 大豆 人碱性成纤维细胞生长因子 |
Detection of Human Basic Fibroblast Growth Factor in the Hairy Roots of Transgenic Soybeans |
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| MA Dong-xue,DONG Guo-gang,YU Jin-lan,HUI Jin-lan,HUI Jin-ling. Detection of Human Basic Fibroblast Growth Factor in the Hairy Roots of Transgenic Soybeans[J]. Progress of Anatomical Sciences, 2009, 15(3): 276-278 | |
| Authors: | MA Dong-xue DONG Guo-gang YU Jin-lan HUI Jin-lan HUI Jin-ling |
| Affiliation: | 1. Pharmacy Department;Air Force General Hospital of PLA;Beijing 100142;2. Department of Radiation;Afficiated Hospital of Air Force in Nanjing Command;Nanjing 200218;3. Air Force 466 Hospital of PLA;Beijing 100089 China |
| Abstract: | Objective To determine the human basic fibroblast growth factor(hbFGF) in the hairy roots of the transgenic soybeans to provide the experimental bases for the mass production of hbFGF making use of hairy roots. Methods Plant genome DNA extraction kit and rabbit anti-human bFGF monoclonal antibody were used to detect the hbFGF in transgenic soybeans' hairy roots by PCR. Results 19 soybeans' hairy roots were proved to contain hbFGF in total sixty Hyg resistance soybean's hairy roots by PCR, positive rate was ... |
| Keywords: | transgene soybean human basic fibroblast growth factor |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |