人牙髓细胞在离体牙髓腔内向成牙本质细胞样细胞分化的研究

目的观察人牙髓细胞在离体牙髓腔内向成牙本质细胞样细胞分化的潜能。方法将原代培养的人牙髓细胞接种至处理过的离体牙髓腔内,2周后固定、脱钙、包埋、切片、染色,显微镜下观察其生长及分化情况。结果牙髓细胞在牙本质表面生长良好,部分细胞伸出胞质突伸入牙本质小管中,表现出成牙本质细胞样细胞的形态。结论牙本质可以诱导牙髓细胞向成牙本质细胞样细胞分化,可为组织工程化牙髓的研制提供实验依据。

Formation of odontoblast-like cells from cultured human dental pulp cells in pulp cavity in vitro

Objective To examine the cell differentiation potential of human dental pulp cells grown in pulp Cavity in vitro. Methods After the pulp cells had been seeded into mechanically and chemically treated pulp cavity for 2 weeks, the samples were fixed, decalcified, embedded, sliced, stained and then observed by microscope. Results The dental pulp cells grew well on dentin surface and some of them exhibited a cytoplasmic process extending into a dentinal tubule, establishing an odontoblast-like morphology on dentin surface in vitro. Dentins can promote the differentiation of dental pulp cells into odontoblast-like ceils, which may offer an experimental basis for the development of tissue engineered pulp.