大鼠肝脏离体再灌注模型的建立及改进

目的 探讨建立大鼠肝脏离体再灌注模型的方法 .方法 SD大鼠肝脏经门静脉和胆道分别插管后切取备用,以加入牛血清白蛋白和牛磺胆酸盐的克-亨(Krebs-Henseleit)液为离体再灌注液.长征-1(CZ-1)型离体再灌注系统的原型包括循环灌注系统和热交换系统2套子系统,改装后省略了热交换系统,循环灌注系统包括1个恒温水浴锅、1个数显恒流泵、1个四口烧瓶、1个液体流量计、1个自制水柱式压力计、1个由玻璃漏斗及玻璃培养皿组成的器官盛放皿、1套铁架台和包括过滤及除泡装置的1套循环管路.用改装后的CZ-1型离体再灌注系统对6只大鼠的肝脏进行120 min的再灌注,灌注过程中检测肝脏胆汁的分泌量,灌注完成后取肝组织于光镜和电镜下进行观察.结果 离体再灌注肝脏的胆汁分泌量为(0.248±0.094)μl·min-1·g-1,再灌注后肝脏组织学和肝细胞的超微结构无明显改变.结论 CZ-1型大鼠肝脏离体再灌注系统价格低廉、结构简单、性能可靠.

Establishment of the isolated perfused rat liver model and its modification

Objective To set up the isolated perfused rat liver model. Methods Rat livers were harvested after the cannulation of the portal vein and bile duct. The reperfusion solution was Krebs-Henseleit solution containing bovine albumin serum and sodium taurocholate. The original CZ-1 isolated perfused rat liver system contained two subsystems: recirculating perfusion system and heat-exchange system. Then we modified the original CZ-1 system and omitted the heat-exchange system.The modified CZ-1 system consisted of a thermostatically regulated water bath,a peristaltic pump,a 4-neck round-bottom flask, a flow meter, an in-line manometer, a glass organ chamber, an iron support and a set of recirculating pipe line. Then the livers were connected via the portal vein to the modified CZ-1 system for 120 min. After 120 min reperfusion, bile production was evaluated. Routine HE staining and electron microscopic examination of hepatic tissues were also performed. Results The was not significantly different from that reported by references. Hepatic tissues in reperfusion group were also morphologically normal Conclusion The CZ-1 isolated perfused rat liver system was cost-effective and reliable to use. It was easy to run and is the ideal model for investigation of organ preservation solution.