HPLC法测定山茱萸生品中5-羟甲基糠醛、莫诺苷和马钱苷含量

目的采用高效液相二极管阵列检测法(HPLC-DAD)同时测定山茱萸生品中3个成分的含量:5-羟甲基糠醛(5-HMF)、莫诺苷和马钱苷。方法采用Venusil ABS C18色谱柱(250 mm×4.6 mm,5μm),以甲醇和水为流动相,梯度洗脱,流速1 ml.min-1,柱温30℃,检测波长284、240、238 nm。结果 5-HMF、莫诺苷和马钱苷的峰面积与浓度的线性关系均良好(r≥0.999 6);加样回收率分别为100%、100.62%、100.53%。结论该方法快速、准确、重现性好,可用于同时测定山茱萸生品中5-羟甲基糠醛、莫诺苷和马钱苷的含量。

Simultaneous determination of 5-HMF,morroniside and loganin in Cornus officinalis by HPLC-DAD

Aim To establish a high performance liquid chromatographic method with diode array detection（HPLC-DAD）for simultaneous determination of 5-HMF,morroniside and loganin in Cornus officinalis.Methods Three constituents were analyzed simultaneously with a Venusil ABS C18 column（250 mm×4.6 mm,5 μm）by gradient elution using methanol-water as the mobile phase.The column was maintained at 30 ℃.The flow rate was 1 ml·min-1.The detection wavelength was set at 284,240 and 238 nm.Results All the constituents showed good linearity（r≥0.9996）in the range of the tested concentration.The average recoveries were 100%,100.62%,100.53%.Conclusion The validated method has the advantages of quickness,precision and reliability,allowing its simultaneous determination of 5-HMF,morroniside and loganin in Cornus officinalis.