| 144例HIV抗体筛查阳性与免疫印迹试验结果对比研究 |
| |
| 引用本文： | 杨积朋,张弛. 144例HIV抗体筛查阳性与免疫印迹试验结果对比研究[J]. 安徽预防医学杂志, 2011, 0(2): 84-85,91 |
| |
| 作者姓名： | 杨积朋 张弛 |
| |
| 作者单位： | 安庆市疾病预防控制中心; |
| |
| 摘 要： | 目的对比分析(ELISA方法检测)H IV抗体试验阳性与免疫印迹试验(WB)结果,探求H IV抗体筛查阳性与确证实验结果的关系。方法对144份ELISA方法检测阳性样本的结果与WB法确证结果进行比较,分析ELISA检测S/CO值变化与WB结果的关系。结果 144例H IV抗体筛查阳性标本经免疫印迹确证试验,阳性的有86例,均为H IV-1抗体阳性,阴性34例,结果不确定的24例。ELISA与WB的阳性符合率为59.7%。确认为阳性标本的S/CO平均值为15.7;不确定的标本S/CO值平均为3.04;阴性的标本S/CO值平均为1.24。在阳性标本中,p55条带出现的频率最低为48.8%,其它均达到85%以上,其中gp160、gp120达到100%;不确定标本中,p24条带出现的几率最高,达75%,其它都在20%以下。结论筛查试验存在假阳性结果,H IV抗体结果的报告必须以确认结果为准。高S/CO值预示H IV抗体阳性的可能性较大,而S/CO值较低时H IV抗体阴性及不确定的可能性较大。WB确认方法在不确定标本检测中存在一定的缺陷。
|
| 关 键 词： | HIV抗体 筛查试验 免疫印迹试验 S/CO值 |
Comparative Analysis of HIV Antibody Screening Positive and Western Blot Test Results |
| |
| YANG Ji-peng,ZHANG Chi. Comparative Analysis of HIV Antibody Screening Positive and Western Blot Test Results[J]. Anhui Journal of Preventive Medicine, 2011, 0(2): 84-85,91 |
| |
| Authors: | YANG Ji-peng ZHANG Chi |
| |
| Affiliation: | YANG Ji-peng,ZHANG Chi(Anqing Center for Disease Control and Prevention,Anqing Anhui,246001,china) |
| |
| Abstract: | Objective To analyze the relationship between ELISA and WB results by comparing the HIV antibody screening positive results and Westem Blot test results.Methods ELISA results of 144 screening positive samples were compared with WB results,the relationship between ELISA and WB results were studied accordingto the variations of ELISA S/CO value.Results There were 86 HIV-1 positive specimens,34 negative specimens,24 indeteminate specimens confirmed by WB test among 144 screening test positive specimens.The pos... |
| |
| Keywords: | HIV antibody Screening test Western Blot test S/CO value |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
