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| 高效液相色谱法测定小柴胡汤中黄芩苷、汉黄芩苷的含量 | |
| 引用本文： | 纪松岗,刘晓帆,朱臻宇,梁珊珊,赵 亮,张 海,柴逸峰.高效液相色谱法测定小柴胡汤中黄芩苷、汉黄芩苷的含量[J].第二军医大学学报,2010,31(9):1010-1013. |
| 作者姓名： | 纪松岗 刘晓帆 朱臻宇 梁珊珊 赵 亮 张 海 柴逸峰 |
| 作者单位： | 1. 解放军401医院药剂科,青岛,266071 2. 第二军医大学药学院药物分析学教研室,上海,200433 3. 第二军医大学东方肝胆外科医院药材科,上海,200438 |
| 摘 要： | 目的 采用高效液相色谱法测定小柴胡汤中黄芩苷、汉黄芩苷的含量。方法 饮片粉末用水回流提取,色谱柱:Agilent Zorbax XDB-C18(150 mm×4.6 mm, 5 μm)；流动相为乙腈和水；A 相为0.2%磷酸-水,B 相为乙腈,梯度洗脱；流速1 ml/min ,柱温25℃,检测波长275 nm,进样量15 μl。结果 黄芩苷和汉黄芩苷在30 min内基线分离。以峰面积(Y)对浓度(X)的标准曲线分别为:黄芩苷Y=44.16X-36.22(r=0.999 9)； 汉黄芩苷Y=52.08X-28.69(r=0.999 9)。日内及日间精密度均小于1%,黄芩苷和汉黄芩苷的定量限分别为0.615 6 μg/ml和0.220 8 μg/ml,黄芩苷和汉黄芩苷加样回收率(n=6)分别为95.73%(RSD=0.8%)及97.02%(RSD=1.56%)。结论 该方法稳定性好,测定结果准确可靠,可用于小柴胡汤的质量控制研究。 |
| 关 键 词： | 高压液相色谱法 小柴胡汤 黄芩苷 汉黄芩苷 含量测定 |
| 收稿时间： | 2010/5/10 0:00:00 |
| 修稿时间： | 2010/6/30 0:00:00 |
High performance liquid chromatography in determination of baicalin and wogonoside contents in Xiaochaihu decoction |
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| JI Song-gang,LIU Xiao-fan,ZHU Zhen-yu,LIANG Shan-shan,ZHAO Liang,ZHANG Hai,and CHAI Yi-feng.High performance liquid chromatography in determination of baicalin and wogonoside contents in Xiaochaihu decoction[J].Academic Journal of Second Military Medical University,2010,31(9):1010-1013. | |
| Authors: | JI Song-gang LIU Xiao-fan ZHU Zhen-yu LIANG Shan-shan ZHAO Liang ZHANG Hai and CHAI Yi-feng |
| Institution: | 1. Department of Pharmacy, No. 401 Hospital of PLA, Qingdao 266071, Shangdong, China;2. Department of Drug Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;3. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China and 2. Department of Drug Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China |
| Abstract: | |
| Keywords: | high performance liquid chromatography Xiaochaihu decoction baicalin wogonoside determination |
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