高效液相色谱-蒸发光散射检测法测定中/长链脂肪乳注射液中磷脂酰胆碱含量

 目的建立高效液相色谱-蒸发光散射检测法测定脂肪乳注射液中磷脂酰胆碱(PC)含量。方法采用Lichrospher 100Diol柱(4.6 mm×250 mm,5μm),以正庚烷-异丙醇(86∶114)为流动相A,正庚烷-异丙醇-水(31∶62∶12)为流动相B,进行梯度洗脱。流速为1.0 mL·min^-1,柱温为40℃;蒸发器温度80℃,空气,喷气流速为2.0 L·min^-1。结果磷脂酰胆碱的线性范围为0.134～0.938 g·L^-1,r=0.998 2(n=7),PC加样回收率为98.9%,RSD为0.3%(n=9)。结论本法操作简便快速、重复性好、灵敏度高、准确、专属性高。

Determination of Phosphatidyi Choline Content in the Medium and Long Chain Fat Emulsion Injection by HPLC-ELSD

OBJECTIVE To develop a HPLC-ELSD method for the determination of phosphatidyl choline(PC) content in the medium and long chain fat emulsion injection.METHODS The separation was performed on a Lichrospher 100 Diol column(4.6 mm×250 mm,5 μm) with n-heptane-iso-propanol(86∶114) as mobile phase A and n-heptane-iso-propanol-water(31∶62∶12) as mobile phase B using linear gradient elution.The flow rate was set at 1.0 mL·min^-1.Column temperature was 40 ℃,inlet heater was 80 ℃.The air flow rate was 2.0 L·min^-1.RESULTS The linear range for the content of PC was 0.134～0.938 g·L^-1,r=0.998 2(n=7).The recovery of PC was 98.9% and its RSD was 0.3%(n=9).CONCLUSION The method is simple,rapid,reproducible,good sensitive,accurate and is suitable for the determination of phosphatidyl choline.