电针对大鼠肠缺血再灌注后炎性损伤的影响

目的:观察电针足三里穴对大鼠肠缺血再灌注炎性损伤的保护作用。方法:48只Wis-tar大鼠随机分为假伤组、模型组、电针组和假针组,每组12只,后3组以阻断肠系膜上动脉45min后再灌注的方法建立肠缺血再灌注大鼠模型。电针组于再灌注前30min施电针(2.5mA,2Hz/100Hz,0.5h)足三里穴治疗,假针组于相同时间内轻轻点刺体表双非经穴点(腹白线旁2cm,约平剑突下5cm水平),假伤组和模型组不予干预治疗。各组于再灌注1h、3h观察肠组织病理损伤程度,检测肠组织含水率、肠组织二胺氧化酶(DAO)活性及肠黏膜血流量(IMBF)的变化。结果:再灌注1h、3h,电针组肠组织病理损伤程度比模型组均明显减轻,肠组织含水率[(74.00±2.11)%、(78.78±0.80)%]比模型组[(80.69±1.66)%、(83.17±2.08)%]显著降低(均P<0.01);肠组织DAO活性[(68.83±4.31)U/L、(47.84±5.57)U/L]和IMBF[(152±5.8)PU、(139.8±6.1)PU]与模型组[(32.86±4.72)U/L、(17.01±2.96)U/L]、[(124.7±8.3)PU、(89.4±13.2)PU]相比均明显升高(均P<0.01);假针组肠组织病理、含水率、DAO活性及IM-BF则均与模型组无显著差异(均P>0.05)。结论:电针不仅能减轻大鼠肠缺血再灌注炎性损伤,还能增加肠组织供血,并能保护由此受损的肠功能。

Effect of electroacupuncture on inflammatory injury induced by intestinal ischemia/reperfusion in rats

Objective To observe the protective effect of electroacupuncture(EA) at Zusanli(ST 36) on inflammatory injury induced by intestinal ischemia/reperfusion(I/R) in rats.Methods Forty-eight Wistar rats were randomly divided into a sham injury group,a model group,an EA group and a sham EA group,12 rats in each group.Intestinal I/R rat models were established by method of clamping with occlusion of superior mesenteric artery(SMA) for 45 min followed by reperfusion.The EA group was treated with EA(2.5 mA,2 Hz/100 Hz,0.5 h) at Zusanli(ST 36) 30 min before reperfusion,and at the same time,the sham EA group was treated with fast insertion at two non-meridian acupoints on skin surface(2 cm horizontally away from linea alba abdominis and about 5 cm paralleled to cartilago ensiformis downward).No interventions were added on the sham injury group and the model group.The degree of pathological injury in intestines,water rate of intestines,diamine oxidase(DAO) activity and intestinal mucosal blood flow(IMBF) were examined at 1 h and 3 h after reperfusion.Results At 1 h and 3 h after reperfusion,the intestinal pathological injury in EA group was significantly attenuated compared with that in model group,and the intestinal water rate of(74.00±2.11)% and(78.78±0.80)% in EA group were significantly lower than(80.69±1.66)% and(83.17±2.08)% in model group(both P<0.01),but DAO of(68.83±4.31)U/L and(47.84±5.57)U/L as well as IMBF of(152±5.8)PU and(139.8±6.1)PU in EA group were significantly higher than DAO of(32.86±4.72)U/L,(17.01±2.96)U/L as well as IMBF of(124.7±8.3)PU and(89.4±13.2)PU in model group(all P<0.01).Meanwhile,the above mentioned changes in sham EA group showed no significant differences compared with those in model group(all P>0.05).Conclusion Electroacupuncture can not only reduce the inflammatory injury induced by intestinal IR but also increase intestinal blood supply so as to protect the intestine function.