HPLC测定四方蒿中总木犀草素苷的含量

 目的建立用反相高效液相色谱法测定四方蒿提取浸膏中主要黄酮苷(木犀草素)含量的方法。方法以ODS C₁₈柱为分析柱,PH 2.0磷酸缓冲液-四氢呋喃-异丙醇(70:30:5)为流动相,检测波长为360nm结果该黄酮水解后的苷元——木犀草素线性关系良好,在高、中、低浓度的日内精密度分别是2.82%,3 41%,5.17%;日间精密度分别是2.56%,5.46%,6.87%; 标准曲线线性0.999 9;检测限为50ng·mL^-1,定量限为(2.40±0.13)μg·mL^-1,方法回收率为高、中、低分别是101.28%, 105.58%,95.56%。结论木犀草素苷含量为(3.12±0.15)% 此法操作简便,准确率、灵敏度高。

Determination of luteolin-glucosides in Elsholtzia blanda by HPLC

OBJECTIVE:To establish a RP-HPLC method for the determination of total luteolin-glucosides content in Elsholtzia blanda Benth extracts (EBBE). METHODS The luteolin content was determined on an ODS C₁₈ column, the mobile phase consisted of 0.01 mol·L^-1 phosphorate buffer (pH 2.0) -tetrahydrofuran-isopropynol (70:30:5) as mobile phase with detection wavelength 360 nm. RESULTS The recoveries of the method were 95.56%～105.58% , and the assay was liner at concentration from 2 to 400 μg·mL^-1(r=0.999 9) .The detection limits was 50 ng·mL^-1(S/N≥3),the limits of quantification was( 2.40±0.13)μg·mL^-1,the content of luteolin in EBBE was (3.12±0.15)%.CONCLUSION This method is simple,accurate and can be used to optimize extraction procedure and determine the content of luteolin-glucosides in Elsholtzia blanda Benth extracts.