茶多酚、吡美莫司、他克莫司外用对莫诺苯宗诱导白癜风样模型小鼠的疗效比较

目的 探讨茶多酚、吡美莫司、他克莫司对莫诺苯宗诱导的白癜风样模型小鼠的疗效差异。 方法 45%莫诺苯宗诱导C57BL/6三周龄雌小鼠脱色，建立白癜风样动物模型。并研究他克莫司、吡美莫司、茶多酚对白癜风样模型小鼠脱色的治疗效果。通过肉眼观察毛发脱色及脱色面积，实验结束后取非用药部位脱色皮肤行组织学检查，HE染色检测淋巴细胞浸润情况，共聚焦激光扫描显微镜（RCM）观察小鼠皮肤的黑素和黑素细胞，免疫荧光检测CD8+ T细胞 。 结果 模型组小鼠在用药部位及非用药部位均有脱色现象。他克莫司组、吡美莫司组、茶多酚组小鼠脱色减少，出现时间晚和面积指数均较模型组低；且用药部位脱色斑局部淋巴细胞和CD8+ T细胞浸润减少。其中他克莫司组疗效优于其他组。 结论 他克莫司、吡美莫司、茶多酚对白癜风样模型小鼠均有疗效。

Comparison of topical application of tea polyphenol versus pimecrolimus versus tacrolimus for the treatment of monobenzone-induced vitiligo-like depigmentation in a mouse model

Li Qing, Zhu Yiping, Xu Ai′e. Department of Dermatology, Third People′s Hospital of Hangzhou, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, ChinaCorresponding author: Xu Ai′e, Email: xuaiehz@msn.com 【Abstract】 Objective To compare the therapeutic effect of topical application of tea polyphenol versus pimecrolimus versus tacrolimus for monobenzone-induced vitiligo-like depigmentation in mice. Methods Twenty-five 3-week-old female C57BL/6 mice were randomly and equally divided into 5 groups: negative control group, model group, tea polyphenol group, pimecrolimus group, tacrolimus group. Monobenzone 45% cream was applied to the back of mice in all the five groups except the negative control group once daily for 40 consecutive days to establish a model of vitiligo-like depigmentation. During the induction of depigmentation, the tea polyphenol group, pimecrolimus group and tacrolimus group were topically treated with tea polyphenol, pimecrolimus and tacrolimus respectively, and the model group remaining untreated. The depigmentation of hairs and skin was observed by naked eyes on a daily basis. Tissue specimens were obtained for histological examination from depigmented skin at non-application sites in mice after the end of the experiment. Hematoxylin-eosin staining was conducted to analyze lymphocytic infiltration, reflectance confocal microscopy to observe melanin and melanocytes in skin, and immunofluorescence assay to detect CD8+ T cell infiltration. Results Depigmentation occurred in both application sites and non-application sites of mice in the model group. Compared with the model group, the tacrolimus, pimecrolimus and tea polyphenol groups showed delayed depigmentation, reduced degree and area index of depigmentation, and attenuated lymphocytic infiltration and CD8+ T cell infiltration in depigmented maculae at application sites. In addition, the therapeutic effect of tacrolimus was stronger than that of pimecrolimus and tea polyphenol. Conclusion Tea polyphenol, pimecrolimus and tacrolimus are all effective for the treatment of vitiligo-like depigmentation in mice.