Omega-3 free fatty acids attenuate insulin-promoted breast cancer cell proliferation |
| |
| Institution: | 1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People''s Republic of China;2. Synergistic Innovation Center for Food Safety and Nutrition, School of Food Science and technology, Jiangnan University, 1800 Lihu Rd, Wuxi 214122, Jiangsu, People''s Republic of China;3. School of Medicine, Jiangnan University, Wuxi, 214122, People''s Republic of China;1. Center for Research and Innovation, Faculty of Medical Technology, Mahidol University, 999 Phutthamonthon 4 Rd, Nakhonpathom 73170, Thailand;2. Institut des Neurosciences Cellulaires et Intégratives, 5 rue Blaise Pascal, Strasbourg 67084, France;3. Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, 25/25 Phuttamonthon 4 Rd, Nakhonpathom 73170, Thailand;4. Chulabhorn Graduate Institute, Chulabhorn Royal Academy, 54 Kamphaeng Phet 6 Rd, Lak Si, Bangkok 10210, Thailand;5. Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, 999 Phutthamonthon 4 Rd, Nakhonpathom 73170, Thailand |
| |
| Abstract: | High insulin levels in obese people are considered as a risk factor to induce breast carcinogenesis. And consumption of fish oils which mainly contain omega-3 fatty acids is associated with a reduced risk of breast cancer. However, whether omega-3 free fatty acids (FFAs) modulate insulin signaling pathway to prevent breast cancer is poorly understood. The current study tested the hypothesis that omega-3 FFAs attenuate insulin-induced breast cancer cell proliferation and regulate insulin signaling pathway. We show here that omega-3 FFAs attenuate MCF-7 cell proliferation and Akt and Erk1/2 phosphorylation levels stimulated by insulin. Knockdown Shp2 by siRNA resulted in significantly elevated omega-3 FFAs–activated Akt phosphorylation but failed to change insulin-stimulated Akt and Erk1/2 phosphorylation. And viable cell number was not affected by either downregulation of Shp2 expression or Erk1/2 inhibitor U0126 treatment. These observations indicated that omega-3 FFAs attenuate insulin-promoted breast cancer cell proliferation and insulin-activated Akt phosphorylation. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
