人结直肠癌中缺氧诱导因子-1α、血管内皮生长因子、生存素表达及意义

目的 探讨缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)、生存素表达在结直肠癌发生发展中的作用以及相互关系.方法 应用免疫组化法检测69例结直肠癌及20例正常肠黏膜中HIF-1α、VEGF、生存素的表达情况,并分析其与结直肠癌临床病理特征之间的关系.结果 HIF-1α、VEGF、生存素在结直肠癌中的表达率分别为56.52%、66.67%、46.38%,而在正常肠黏膜则表达不明显或不表达,两者差异有统计学意义(P＜0.01).HIF-1α、VEGF及生存素的表达与结直肠癌分化程度、肿瘤浸润深度、淋巴结转移和临床分期均密切相关(P＜0.05).HIF-1α蛋白的表达与VEGF及生存素蛋白的表达均呈显著正相关,VEGF与生存素的表达亦呈显著正相关.结论 HIF-1α通过上调VEGF及生存素表达促进结直肠癌的发生发展;VEGF与生存素间存在协同作用.

Expression and clinical significance of hypoxia-inducible factor-1α，vescular endothelial growth factor and survivin in colorectal cancer

Objective To evaluate the expressions of hypoxia-inducible factor-1α(HIF-1α),vescular endothelial growth factor(VEGF)and survivin in developing colorectal cancer,and the association among them.Methods The protein expressions of HIF-1α,VEGF and survivin were detected in specimens obtained from 69 patients with colorectal cancer and 20 normal controls by immunohistochemistry.The correlations of HIF-1α,VEGF and survivin with clinicopathologic features were analyzed. Results The expression of HIF-1α,VEGF and survivin proteins in patients with colorectal cancer were 56.52%,66.67% and 46.38%,respectively,and little expressed in normal controls(P＜0.01).The expressions of HIF-1α,VEGF and survivin were closely related to the differential grade of adenocarcinoma,he in volvment of penetration,lymph node metastasis and Dukes stage(P＜0.05).In addition,HIF-1α protein expression had positive correlations with both VEGF and survivin(P＜0.05),and the expression of VEGF also had positive correlation with survivin(P＜0.05).Conclusions HIF-1α may involve in the pathogenesis of colorectal cancer through up regulating the expressions of VEGF and survivin,which may have synergetic effects in the pathogenesis of colorectal cancer.