基于双向电泳技术的体液蛋白质组学研究进展

目前 ,蛋白质组技术被大量应用于临床和生物学研究各个领域 ,其中的大部分研究都在双向电泳技术的支持下展开。体液样品 (包括血浆、脑脊液、尿液等 )蛋白质的全面分析主要依赖于蛋白质组学分析。本文综述了基于双向电泳技术的体液蛋白质组学研究中样品制备、生物标志物探索、药物效应评价和化合物毒性分析等方面的研究进展

Research advances of two-dimensional gel electrophoresis baesed proteomic application in biological fluids

Proteomics is one of the technologies rapidly used in many branches of biomedical sciences. The application of proteomics is mainly based on two dimensional electrophoresis(2 DE). Although both mRNA and proteomic profiling can be applied to tissue samples, analysis of biological fluids(e.g. serum, CSF, urine) is restricted to proteomics. This review summarizes recent advances in proteomic application in biological fluids , with emphasis on sample preparation, biomarker discovery, as well as evaluation of drug effects and toxicity.