蒙古黄芪的化学成分研究

 目的研究豆科黄芪属植物蒙古黄芪[Astragalus membranaceus(Fish.)Bge.var.mongholicus(Bge.)Hsiao]根中的化学成分。方法采用体积分数为80%乙醇提取,硅胶柱色谱分离及重结晶等方法从蒙古黄芪中分离化合物,根据理化性质和波谱数据鉴定化合物结构。结果分离并鉴定了17个化合物,其中7个为皂苷类化合物,分别为:黄芪皂苷Ⅳ(ZD₁),异黄芪皂苷Ⅱ(ZD₂),黄芪皂苷Ⅱ(ZD₃),膜荚黄芪皂苷Ⅱ(ZD₄),黄芪皂苷Ⅰ(ZD₅),乙酰黄芪皂苷Ⅰ(ZD₆),异黄芪皂苷Ⅰ(ZD₇);2个为异黄烷类化合物,分别为:2′,4′-二甲氧基-3′-羟基异黄烷-6-O-β-葡萄糖苷(ZD₁₀),(3R)-8,2′-二羟基-7,4′-二甲氧基异黄烷(ZD₁₄);2个为紫檀烷类化合物,分别为:(6aR,11aR)9,10-二甲氧基紫檀烷-3-O-β-D-葡萄糖苷(ZD₁₁),(6aR,11aR)10-羟基-3,9-二甲氧基紫檀烷(ZD₁₂);4个为异黄酮类化合物,分别为:芒柄花素-7-O-β-D-葡萄糖苷(ZD₈),芒柄花素-7-O-β-D-葡萄糖苷(ZD₁₆),毛蕊异黄酮(ZD₁₃),阿弗罗摩辛(ZD₁₇);其余2个化合物为:β-谷甾醇(ZD₉)和胡萝卜苷(ZD₁₅)。结论化合物ZD₂,ZD₄,ZD₆,ZD₇,ZD₁₀,ZD₁₄为首次从该种植物中分得。

Studies on Chemical Constituents of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao

OBJECTIVE To study the active constituents of Astragalus membranaceus(Fisch.) Bge.var.mongholicus(Bge.) Hsiao.METHODS Various chromatographic techniques were used to isolate and purify the constituents,their physico-chemical properties and spectral data were determined to elucidate the structures.RESULTS Seventeen compounds were isolated and identified as: astragaloside IV(ZD₁),isoastragaloside Ⅱ(ZD₂),astragaloside Ⅱ(ZD₃),astramembrannin Ⅱ(ZD₄),astragaloside Ⅰ(ZD₅),acetylastragaloside Ⅰ(ZD₆),isoastragaloside I(ZD₇),formononetin(ZD₈),β-sitosterol(ZD₉),2′,4′-Dimethoxy-3′-hydroxy-isoflavan-6-O-β-glucopyranoside(ZD₁₀),(6aR,11aR)9,10-dimethoxypteroca-rpan-3-O-β-D-glucoside(ZD₁₁),(6aR,11aR)3,9-dimethoxy-10-hydroxypterocarpan(ZD₁₂),calycosin(ZD₁₃),(3R)8,2′-dihydroxy-7,4′-dimethoxyisoflavan(ZD₁₄),daucosterol(ZD₁₅),formononetin-7-O-β-D-glucoside(ZD₁₆),afrormosin(ZD₁₇).CONCLUSION Compound ZD₂,ZD₄,ZD₆,ZD₇,ZD₁₀ and ZD₁₄ are isolated from this plant for the first time.