血清淀粉样P蛋白缺失对小鼠动脉粥样硬化斑块形成的影响

目的观察血清淀粉样P蛋白(serum amyloid P component,SAP)缺失对小鼠动脉粥样硬化形成的影响。方法构建载脂蛋白E(Apolipoprotein E)和SAP双基因敲除小鼠(ApoE-/-;SAP-/-)作为实验组,ApoE-/-小鼠作为对照组。2组小鼠各12只,组内雄雌小鼠各半。在小鼠第8周龄时,体质量约20 g,此时开始同时给予2组小鼠高脂饮食,于第16周龄时获取小鼠全血分离得到血清进行血脂分析,处死小鼠后获取其心脏,胸腹主动脉进行油红O染色并进行病理统计。结果双基因敲除小鼠组的高密度脂蛋白胆固醇(HDL-C)明显高于对照小鼠(P<0.01),低密度脂蛋白和高密度脂蛋白的比值和动脉粥样硬化指数(AI)小于对照组小鼠且差异显著(P<0.01),而总胆固醇(TC),甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)浓度无显著性差异(P>0.05);主动脉根部切片和胸腹主动脉的油红O染色结果表明SAP缺失明显减轻了小鼠动脉粥样硬化斑块面积(P<0.01)。结论 AP缺失可能通过上调HDL从而抑制ApoE-/-小鼠动脉粥样硬化斑块形成。

Effect of serum amyloid P component deficiency on formation of atherosclerosis plaques in mice

Objective To observe the effect of serum amyloid P component(SAP) deficiency on atherosclerosis(AS)in mice.Methods Apolipoprotein E(ApoE) and SAP knockout mice served as an experimental group(n=12) and ApoE knockout mice served as a control group(n=12).Their average body weight was about 20 g at the age of 8 weeks.The animals were then fed with high fat diet and killed at the age of 16 weeks.Blood,heart,and thoracico-abdominal aorta tissue samples were taken.Serum lipid was analyzed and the AS lesion Plaque area was measured after oil red O staining.Results The HDL-C level was significantly higher while the LDL-C/HDL-C value and atherogenic index(AI)were significantly lower in experimental group than in control group(P<0.01).However,no significant difference was observed in TC,TG and LDL-C levels between the two groups(P>0.05).Oil red O staining showed that SAP deficiency significantly reduced the area of atherosclerosis plaques in heart and thoracico-abdominal aorta tissue samples(P<0.01).Conclusion SAP deficiency inhibits formation of atherosclerosis plaques in mice by up-regulating their HDL-C level.