Leishmania species identification using FTA card sampling directly from patients' cutaneous lesions in the state of Lara, Venezuela |
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| Authors: | Kato Hirotomo Watanabe Junko Mendoza Nieto Iraida Korenaga Masataka Hashiguchi Yoshihisa |
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| Affiliation: | aLaboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan;bJapan Overseas Cooperation Volunteers (JOCV), Japan International Cooperation Agency (JICA), Tokyo, Japan;cThe Japanese Red Cross College of Nursing, Tokyo, Japan;dServicio de Dermatología Sanitaria Estado, Edificio de Inmunologia, Hospital Central, Estado de Lara, Venezuela;eDepartment of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan |
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| Abstract: | ![]()
A molecular epidemiological study was performed using FTA card materials directly sampled from lesions of patients with cutaneous leishmaniasis (CL) in the state of Lara, Venezuela, where causative agents have been identified as Leishmania (Viannia) braziliensis and L. (Leishmania) venezuelensis in previous studies. Of the 17 patients diagnosed with CL, Leishmania spp. were successfully identified in 16 patients based on analysis of the cytochrome b gene and rRNA internal transcribed spacer sequences. Consistent with previous findings, seven of the patients were infected with L. (V.) braziliensis. However, parasites from the other nine patients were genetically identified as L. (L.) mexicana, which differed from results of previous enzymatic and antigenic analyses. These results strongly suggest that L. (L.) venezuelensis is a variant of L. (L.) mexicana and that the classification of L. (L.) venezuelensis should be reconsidered. |
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| Keywords: | Venezuela Leishmania venezuelensis Leishmania mexicana Leishmania braziliensis Cytochrome b rRNA internal transcribed spacer |
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