胆管癌中TWIST蛋白、Kiss-1蛋白和上皮黏附素的表达及临床意义

目的 探讨胆管癌中TWIST、Kiss-I和上皮黏附素（E-cadherin）的表达,观察三者在胆管癌发生、发展及转移中的作用,以期为临床工作提供理论帮助.方法 收集109例胆管癌标本作为观察组,收集70例正常胆管上皮组织作为对照组,应用免疫组织化学技术检测胆管癌中TWIST、Kiss-1和E-cadherin蛋白的表达,分析三者在不同临床特征中的表达差别及相关性.结果 TWIST在胆管癌组织中高表达（54.13％比15.71％）,Kiss-1（31.19％比95.71％）和E-cadherin（27.52％比91.43％）（P＜0.01）在胆管癌组织中低表达,TWIST、Kiss-1和E-cadherin在不同分化、不同浸润深度、不同淋巴结转移患者中的表达有明显的差异（P＜0.05）,观察组中TWIST、Kiss-1和E-cadherin的表达具有相关性（P＜0.05）.结论 TWIST、Kiss-1和E-cadherin异常表达在胆管癌发生发展中起重要作用,三者具有协同效应,共同促进肿瘤的进展.

Expression and clinical significance of TWIST protein, Kiss-1 protein and epithelial adhesin in cholangiocarcinoma

Objective To investigate the expression of TWIST,Kiss-1 and epithelial adhesin （E-cadherin） in cholangiocarcinoma and observe their role in the occurrence,development and metastasis of cholangiocarcinoma in order to provide theoretical help for clinical work.Methods 109 cases of cholangiocarcinoma specimens were assigned to the observation group and 70 cases of normal bile duct epithelial tissue were assigned to the control group.The expression of TWIST,Kiss-1 and E-cadherin proteins were detected using the immunohistochemical technique.The expression difference and correlation of the three proteins in different clinical characteristics were analyzed.Results TWIST was highly expressed in cholangiocarcinoma （54.13％ vs 15.71％） while Kiss-1 （31.19％ vs 95.71％） and E-cadherin （27.52％ vs 91.43％） were lowly expressed （P ＜ 0.01）.The expression of TWIST,Kiss-1 and E-cadherin were significantly different in the patients with differentiation,different depth of invasion and different lymph node metastasis （P ＜0.05）.In the observation group,the expression of Twist,Kiss-1 and E-cadherin were correlated （P ＜ 0.05）.Conclusion The abnormal expression of TWIST,Kiss-1 and E-cadherin play important role in the development of cholangiocarcinoma.The three proteins have a synergistic effect and jointly promote the tumor progress.