| EFFECT OF ADENOVIRUS—MEDIATED p53 GENE TRANSFER ON APOPTOSIS AND RADIOSENSITIVITY OF HUMAN |
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| 引用本文： | 张珊文,肖绍文,吕有勇.EFFECT OF ADENOVIRUS—MEDIATED p53 GENE TRANSFER ON APOPTOSIS AND RADIOSENSITIVITY OF HUMAN[J].中国癌症研究,2003,15(1):14-18. |
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| 作者姓名： | 张珊文 肖绍文 吕有勇 |
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| 作者单位： | DepartmentofRadiationOncology,BeijingUniversitySchoolofOncology,Beijing100036 |
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| 基金项目： | This work was supported by the National Natural Science Foundation of China (No. 39670234). |
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| 摘 要： | To evaluate the effect of adenovirus-mediated p53 gene(Adp53) on apoptosis and radiosensitivity of human gastric carcinoma cell lines.Methods:Recombinant adenovirus expressing wild-type p53 lines with different p53 genetic status.p53 protein expression was detected by immunohistochemistry assay and western blot assay.Cell survival was assessed using a clonogenic assay.TUNEL assay was used in determination of apoptosis.Four human gastric carcinoma cells infected with Adp53 were irradiated with 4Gy and cell cycle distribution and Sub-G1 peak were assayed by flow cytometry.Results:G2/M arrest,apoptosis and inhibition of tumor cell proliferation were induced by infection at Adp53 at 100 MOI which caused high transfer rate of wild-type p53 and strong expression of p53 protein in four human gastric carcinoma cells.The radio-enhancement ratio of Adp53 at 4Gy were3.0 for W cell,3.6 for M cell,2.2 for neo cell and 2.5 for 823 cell in vitro.Conclusion :This study demonstrated that Adp53 transfer increased cellular apoptosis and radiosensitivity of human gastric carcinoma cell lines in vitro independently on cellular intrinsic p53 status thus supporting the combination of p53 gene therapy with radiotherapy in clinical trials.
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| 关 键 词： | 人胃癌细胞系 腺病毒介导 p53基因转染 肿瘤细胞凋亡 放疗敏感性 |
| 收稿时间： | 23 November 2002 |
| 修稿时间： | 23 January 2003 |
Effect of adenovirus-mediated p53 gene transfer on apoptosis and radiosensitivity of human gastric carcinoma cell lines |
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| Zhang?Shan-wen?Email author,Xiao?Shao-wen,Lü?You-yong.Effect of adenovirus-mediated p53 gene transfer on apoptosis and radiosensitivity of human gastric carcinoma cell lines[J].Chinese Journal of Cancer Research,2003,15(1):14-18. |
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| Authors: | Email author" target="_blank">Zhang?Shan-wen?Email author Xiao?Shao-wen Lü?You-yong |
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| Institution: | (1) Department of Radiation Oncology, Beijing University School of Oncology, 100036 Beijing |
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| Abstract: | Objective: To evaluate the effect of adenovirus-mediated p53 gene (Adp53) on apoptosis andradiosensitivity of human gastric carcinoma cell lines.Methods: Recombinant adenovirus expressing wild-type p53 gene was transferred into four human gastric carcinoma cell lines with different p53 genetic status. p53 proteinexpression was detected by immunohistochemistry assayand western blot assay. Cell survival was assessed using a clonogenic assay. TUNEL assay was used in determination of apoptosis. Four human gastric carcinoma cells infectedwith Adp53 were irradiated with 4Gy and cell cycle distribution and Sub-G1 peak were assayed by flowcytometry. Results: G2/M arrest, apoptosis and inhibition of tumor cell proliferation were induced by infection atAdp53 at 100 MOI which caused high transfer rate ofwild-type p53 and strong expression of p53 protein in four human gastric carcinoma cells. The radio-enhancement ratio of Adp53 at 4Gy were 3.0 for W cell, 3.6 for M cell, 2.2 for neo cell and 2.5 for 823 cell in vitro. Conclusion: Thisstudy demonstrated that Adp53 transfer increased cellularapoptosis and radiosensitivity of human gastric carcinoma cell lines in vitro independently on cellular intrinsic p53status thus supporting the combination of p53 gene therapy with radiotherapy in clinical trials. |
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| Keywords: | Gastric carcinoma Radiosensitivity Apoptosis Adenovirus-mediated p53 gene |
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