Dicer1在宫颈肿瘤组织中的表达及意义

目的:探讨Dicer1在正常宫颈上皮、宫颈上皮内瘤变和宫颈鳞癌的表达及意义。方法:Western blot和RT-PCR法检测正常细胞系(End1/E6E7)、宫颈内瘤变细胞系(S12)及宫颈癌细胞系(Ca Ski、He La、Si Ha和C33A)中Dicer1蛋白及其mRNA表达水平,免疫组织化学技术检测Dicer1在正常宫颈、宫颈上皮内瘤变和宫颈鳞癌中的表达水平。结果:正常宫颈上皮细胞系Dicer1表达水平低于宫颈癌细胞系表达量。随着恶性程度增加,Dicer1呈驼峰状表达,即在正常宫颈组织及Grade III级宫颈鳞癌中表达较低,但在宫颈上皮内瘤变及高分化宫颈肿瘤(grade I级)中表达较高。结论:Dicer1在正常宫颈、宫颈上皮内瘤变和宫颈鳞癌中的表达有差异,为进一步研究Dicer1在宫颈癌发生发展中的作用及分子机制提供依据。

The expression of Dicer1 in the cervical cancer and normal cervical epithelial tissues

Objective:To evaluate the expression of Dicer1 in normal cervix,precancerous cervical lesions and cervical cancer,and investigate the significance of Dicer1 expression in the development of cervical cancer.Methods:The mRNA and protein expression of Dicer1 in cervical cancer cells were analyzed by RT-PCR and Western blot,respectively.The expression of Dicer1 in cervical cancer tissues and normal cervical epithelial tissues was detected by IHC,the levels of Dicer1 expression were evaluated by pathologist visual scoring.Results:The Dicer1 expression in cervical cancer and precancerous cervical cells were up-regulated compared with the End1/E6E7 cells.In accordance with cell lines,the Dicer1 was also overexpressed in cervical lesions tissues.Furthermore,the expression of Dicer1 was decreased gradually by the pathological grades.Conclusion:The expression of Dicer1 in cervical lesions was associated with the pathological grades.The Dicer1 expression in cervical cancer may contribute to tumor initiation and progression.