硫代乙酰胺剂量个体化诱导大鼠肝硬化门脉高压模型

目的 探索根据大鼠对硫代乙酰胺(thioacetamide,TAA)反应的个体差异,采用剂量个体化诱导肝硬化门静脉高压模型,以提高成模率和模型质量.方法 50只雄性SD大鼠随机分为3组,第1组(20只),按照传统方法诱导大鼠肝硬化.第2组(20只),剂量个体化诱导大鼠肝硬化.第3组(10只)为对照组.结果 第1组大鼠死亡率为30%(6/20),肝硬化形成率为50%(10/20);第2组死亡率为10%(2/20),肝硬化形成率为80%(16/20);对照组全部存活.与对照组相比第2组门静脉压力、脾髓压力明显增高,平均动脉压显著降低(P＜0.05);总胆红素、谷丙转氨酶显著升高(P＜0.05);血清白蛋白、红细胞、血红蛋白及血小板显著降低(P＜0.05).结论 肝硬化诱导过程中根据大鼠对TAA反应的个体差异,调整TAA的诱导剂量,可成功诱导大鼠肝硬化门静脉高压,与传统制模方法相比,该法明显降低大鼠死亡率,并显著提高肝硬化形成率和质量.

Model of rat's liver cirrhosis and portal hypertension induced by individuation of thioacetamide dosage

Objective To develop an ideal means to induce rat's liver cirrhosis and portal hypertension by individuation of Thioacetamide(TAA) dosage based on rat's variations in response to TAA.Methods Fifty male SD rats were divided into three groups.Group 1(n=20) was treated with traditional means.Group 2(n=20) was treated with the dose modified according to weight changes during the induction of cirrhosis.Group 3(n=10) was control group.Results The mortality in group 1 was 30%(6/20) and cirrhosis developed was 50%(10/20).In contrast,the mortality in group 2 was 10%(2/20) and cirrhosis developed was 80%(16/20).The control group was all survival.The PVP and SPP of group 2 were higher than that of group 3 and MAP was lower than that of group 3(P<0.05).STB and ALT were higher than that of group 3,but SAB,RBC,HB and PLT decreased obviously(P<0.05).Conclusion Adjusting the inducible of TAA dosage based on rats' variations in response to TAA can successfully induce rat's liver cirrhosis and portal hypertension.Compared with the traditional method,our method can obviously reduce mortality and strikingly increase the production and quality of cirrhosis induced in the rat.