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| 国内外真菌毒素检测方法研究现状及进展 | |
| 引用本文： | 李世雨, 傅强, 严亚贤. 核酸适配体在真菌毒素检测中研究进展[J]. 中国公共卫生, 2018, 34(4): 618-622. DOI: 10.11847/zgggws1115739 |
| 作者姓名： | 李世雨 傅强 严亚贤 |
| 作者单位： | 1.上海交通大学农业与生物学院 上海市兽医生物技术重点实验室，上海 200240 |
| 基金项目： | 国家自然科学基金（31571932） |
| 摘 要： |  核酸适配体是指在体外利用指数富集的配基系统进化技术筛选得到的核酸（DNA或RNA）片段。基于核酸适配体的检测方法具有特异性高、稳定性好、易于修饰等优点，在真菌毒素检测领域得到了广泛的关注和应用。本文在传统检测方法的基础上，着重介绍核酸适配体在真菌毒素检测领域中的研究进展，并提出目前存在的问题及发展趋势。  |
| 关 键 词： | 核酸适配体 真菌毒素 检测 |
| 收稿时间： | 2017-06-20 |
Simultaneous detection of multiple mycotoxins in broiler feeds using a liquid chromatography tandem-mass spectrometry |
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| Shi-yu LI, Qiang FU, Ya-xian YAN. Progress in researches on utilization of aptamers in mycotoxin detection[J]. Chinese Journal of Public Health, 2018, 34(4): 618-622. DOI: 10.11847/zgggws1115739 | |
| Authors: | Shi-yu LI Qiang FU Ya-xian YAN |
| Affiliation: | 1.Shanghai Municipal Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China |
| Abstract: | Aptamers are single stranded DNA or RNA molecules obtained with systematic evolution of ligands by exponential enrichment (SELEX). The detection method based on aptamers draws extensive attentions and applications in the field of mycotoxin detection due to many obvious advantages such as high specificity, good stability and easy modification. This article describes the latest progress of aptamer utilization in mycotoxin detection and proposes the existing problems and development trend based on the conventional detection method. |
| Keywords: | aptamer mycotoxin detection |
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