Identification and enhancing production of a novel macrolide compound in engineered Streptomyces peucetius |
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| Authors: | Van Thuy Thi Pham Hue Thi Nguyen Chung Thanh Nguyen Ye Seul Choi Dipesh Dhakal Tae-Su Kim Hye Jin Jung Tokutaro Yamaguchi Jae Kyung Sohng |
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| Affiliation: | Department of Life Science and Biochemical Engineering, SunMoon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si Chungnam 31460 Republic of Korea.; Department of Pharmaceutical Engineering and Biotechnology, SunMoon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si Chungnam 31460 Republic of Korea |
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| Abstract: | ![]()
Streptomyces peucetius produces doxorubicin and daunorubicin, which are important anticancer drugs. In this study, we activate peucemycin, a new antibacterial compound, using an OSMAC strategy. In general, bioactive compounds are produced in a higher amount at room temperature; however, in this study, we have demonstrated that a bioactive novel compound was successfully activated at a low temperature (18 °C) in S. peucetius DM07. Through LC-MS/MS, IR spectroscopy, and NMR analysis, we identified the structure of this compound as a γ-pyrone macrolide. This compound was found to be novel, thus named peucemycin. It is an unusual 14-membered macrocyclic γ-pyrone ring with cyclization. Also, peucemycin exhibits potential antibacterial activity and a suppressive effect on the viability of various cancer cell lines.Activation of peucemycin in S. peucetius DM07 by the OSMAC strategy. |
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