欣怡胶囊对兔急性心肌梗死再灌注后心肌无复流程度的影响

目的：观察欣怡胶囊对兔急性心肌梗死（AMI）再灌注后心肌无复流的影响。方法将80只兔分为5组,模型组,阳性药组（替罗非班60μg/kg）,欣怡胶囊高、中、低剂量组（4.0 g生药/kg、2.0 g生药/kg、1.0 g生药/kg）,每组16只,另取14只作为假手术组,灌胃给药5 d后分别建立 AMI缺血再灌注模型,结扎兔冠状动脉左回旋支近段3 h,再灌注1 h,记录 AMI术前和 AMI再灌注后心电图,比较 J点抬高情况；AMI再灌注后左心室插管连接 POWERLAB生物信号采集处理系统,记录血流动力学相关指标；采用Evan’s蓝和硫磺素 S对心脏进行活体染色,记录心肌无复流程度。结果欣怡胶囊高、中和低剂量组心肌无复流程度均比模型组显著降低,分别降低了38.1%、24.5%和19.7%（P〈0.05或P〈0.01）,AMI再灌注后心电图中 J点比模型组明显降低,左心室内压（LVSP）、心率（HR）、左心室压力最大上升速率（＋dp/dtmax）、左心室内压最大下降速率（-dp/dtmax）明显升高,左心室舒张末期内压（LVEDP）明显降低,差异有统计学意义（P〈0.05或P〈0.01）。结论欣怡胶囊灌胃给药能一定程度预防和改善兔 AMI再灌注后导致的心肌无复流现象。

Effect of Xinyi Capsule on No Reflow After Reperfusion of Acute Myocardial Infarction in Rabbits

Objective To investigate the effect of Xinyi capsule（XYC）on no reflow（NR）after reperfusion of acute myocardial infarction（AMI）in rabbits.Methods Ninety four rabbits were enrol ed in this study.We randomly al ocated 80 rabbits and divided them into 5 groups randomly（n= 16）,model group,positive drug group（Tirofiban 60μg/kg）,XYC high dose group,XYC medium dose group,XYC low dose group（4.0 g,2.0 g,1.0 g crude drug /kg）.14 rabbits were classified as sham operation group.After 5 days of intragastric administration with drug,AMI ischemia reperfusion model was created by ligating proximal left circumflex coronary ar-tery for 3 hours,and fol owed by myocardial ischemia reperfusion for 1 hour.Recordings of electrograms before interventional oper-ation and after reperfusion of AMI were performed,the degree of J point elevation were compared among the recordings;after reper-fusion of AMI,left ventricular hemodynamic parameters were measured by PowerLab biological signal col ection and processing sys-tem;the degree of NR were recorded by the method of vital staining with Evan’s blue and Thioflavin S.Results Compared with the model group,the degree of NR was significantly decreased at 38.1%,24.5%,19.7% in XYC high dose group,medium dose group, low dose group,respectively（P〈0.05 or P〈0.01）.After reperfusion of AMI,the J point of the electrogram lowered significantly. LVSP,HR,±dp/dtmax increased significantly while LVEDP reduced significantly（P〈0.05 or P〈0.01）.Conclusion XYC could pre-vent and treat the NR phenomenon resulted from reperfusion of AMI in rabbits to some degree.