清解颗粒质量标准研究

目的：建立清解颗粒质量标准。方法：采用TLC法对处方中的连翘、夏枯草、牡丹皮、蒲公英、柴胡进行了定性鉴别，并用HPLC法对处方中连翘苷进行了含量测定，采用Phenomenex Gemini 5μm C18色谱柱，以乙腈-水（22：78）为流动相，检测波长为228nm。结果：薄层色谱斑点清晰，易于识别，专属性强；连翘苷在0．05948～t．1896μg呈良好的线性关系（r=0．9999），平均回收率为98．4％，RSD为1．7％。结论：该方法准确灵敏、简便，重复性好，提高后的质量标准更有效的控制该制剂的质量。

Studies on Quality Standard of Qingjie Granules

Objective： To determine the quality standard of Qingjie Granules. Methods： Fructus forsythiae, Sica prunellae, Cortex montan, Herba taraxaei, Radix bupleuri in Qingjie Granules were identified by TLC. The content of Forsythin was determined by HPLC . The chromatographic conditions were： Phenomenex Gemini C18 （4. 6 mm×250 mm, 5 μm）column, mobile phase being acetonitrile- water （22： 78）, and the detection wavelength at 228 nm. Results： The qualitative identification with TLC were specific. Forsythin showed a good linearity within the range of 0. 059 48 - 1. 189 6 μg, r = 0. 999 9, the average recovery was 98.4%, and RSD was 1.7% . Conclusion： This method can be used for the quality control of Qingjie Granules.