| 乙型肝炎病毒DNA检测与其血清标志物的相关性 |
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| 引用本文： | 梁亚莉,王卫萍,唐风,邵海枫,史利宁,胡毓安,陈勇.乙型肝炎病毒DNA检测与其血清标志物的相关性[J].中国热带医学,2009,9(12):2224-2224,2266. |
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| 作者姓名： | 梁亚莉 王卫萍 唐风 邵海枫 史利宁 胡毓安 陈勇 |
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| 作者单位： | 1. 南京军区南京总医院临床中心实验科,江苏,南京,210002;扬州友好医院检验科,江苏,扬州,225261
2. 南京军区南京总医院临床中心实验科,江苏,南京,210002 |
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| 摘 要： | 目的探讨实时荧光聚合酶链反应（FIQ—PCR）检测HBV—DNA与乙型肝炎病毒五项血清标志物（HBV—M）的关系。方法采用FO—PCR技术和酶联免疫吸附法（ELISA）分别检测637例患者血清中的HBV—DNA拷贝数和乙肝五项血清标志物。结果HBV—DNA在“大三阳”组（HBsAg＋HBeAg＋抗HBc＋和HBsAg＋HBeAg＋）和“小三阳”组（HBsAg＋抗HBe＋抗HBc＋和HBsAg＋抗LHBc＋）可检出，在“单纯抗体阳性”组（抗HBs＋抗HBe＋抗HBc＋、抗HBs＋抗HBc＋、抗HBs＋抗HBe＋、抗HBs＋、抗HBe＋抗HBc＋及抗HBc＋）未捡出。“大三阳”组患者血清中HBV—DNA检出率为92．13％。且82．68％的患者HBV—DNA拷贝数在105copies／ml以上；“小三阳”组患者血清中HBV—DNA检出率为44．39％，HBV—DNA拷贝数分布范围广。结论HBeAg的存在可间接反映HBV在体内的复制状态，而HBV—DNA的检测更客观地反映了HBV复制的程度和传染性。
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| 关 键 词： | 乙型肝炎病毒血清标志物（HBV—M） HBV—DNA 实时荧光聚合酶链反应（FQ—PCR） 酶联免疫吸附试验（EUSA） |
Correlation of HBV-DNA detection and HBV serological markers |
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| Institution: | LIANG Ya-li, WANG Wei -ping, TANG Feng, et al.(Institute of Chnical Laboratory Medicine,Nanjing General Hospital of Nanjing Mihtary Garrison,Nanjing 210002,P.R. China ) |
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| Abstract: | Objective To investigate the relationship between HBV-DNA detection and HBV serological markers. Methods The content of HBV-DNA and serological markers of HBV were detected with real-time. FQ-PCR and ELISA respectively. Results HBV-DNA was detected from groups positive for HBsAg,HBeAg,HBcAb and HbsAg, HBeAb and HBcAb,ant it was not detected in other groups. The positive rate of detectable HBV-DNA in groups positive for HBsAg positive,HbeAg,HbcAb was 92.13%. The positive rate of detectable HBV-DNA in groups positive for HbsAg, HbeAb,HBcAb was 44.39%. Conclusions The detection of HBV-DNA indirectly indicates the status of infection, replication. |
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| Keywords: | HBV-DNA |
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