| 大鼠局灶性脑缺血早期脑内HO-1 蛋白的表达 |
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| 引用本文： | 符荣,陈衔城,任惠民,宋后燕,金复生,季耀东. 大鼠局灶性脑缺血早期脑内HO-1 蛋白的表达[J]. 复旦学报(医学版), 2002, 29(1): 53-56,F003 |
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| 作者姓名： | 符荣 陈衔城 任惠民 宋后燕 金复生 季耀东 |
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| 作者单位： | 复旦大学附属华山医院神经外科,上海,200040;复旦大学附属华山医院神经病学研究所,上海,200040;复旦大学附属华山医院医学院分子遗传学研究室,上海,200040;复旦大学附属金山医院中心实验室 上海,200540 |
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| 基金项目： | 复旦大学优秀博士学位论文培育资助项目 (CQF15 180 1) |
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| 摘 要： | 目的 研究局灶性脑缺血血红素氧合酶 (HO)在脑内的表达。方法 采用大鼠大脑中动脉栓塞致脑缺血 ,对缺血早期不同时间点进行HO 1免疫组化及病理学研究 ,并应用计算机图像分析技术计算HO 1表达的强弱。结果 栓塞后 3 0min皮质及海马即有HO 1阳性神经元和胶质细胞 ,且随着时间推移 ,HO 1的表达逐渐增强 ,HO 1主要分布在灶周皮质、梗死皮质区、梨状皮质内的神经元及胶质细胞 ,丘脑、下丘脑、海马齿状回的神经元及对测海马也出现HO 1的表达。结论 脑缺血时脑内HO 1迅速而强烈的表达可能是脑组织对自身损伤的恢复机制
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| 关 键 词： | 局灶性脑缺血 一氧化碳 血红素氧合酶-1 免疫组化 |
Neuronal Expression of Heme Oxygenase Protein During Early Focal Cerebral Ischemia in Rats |
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| Abstract: | Purpose To study the expression of heme oxygenase protein during early focal ischemia in rats. Methods The expression of heme oxygenase(HO) 1 protein during early focal cerebral ischemia was investigated by using immunohistochemistry and computer image analysis in middle cerebral artery occlusion in rats. Results HO??1 expression was observed in the neurons and gliacytes of the ipsilateral cerebral cortex infarction area and hippocampus at 30 miniute after occlusion.With time going on ,the expression of HO?? 1 increased,HO??1 expression was observed in the ipsilateral infarction border area,frontal and cingulated cortex,thalamus,hypothalamus and bilateral hippocampus. Conclusions Strong and long lasting expression of HO??1 protein is evidently significant physiological consequences in the recovery of neuronal tissue from ischemic injury in focal cerebral ischemia. |
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| Keywords: | focal cerebral ischemia carbon monoxide heme oxygenase 1 immunohistochemistry |
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