1983-2013年B型流感病毒的重配研究

目的 研究1983-2013年间B型流感病毒的重配变异情况.方法 对GenBank数据库中1983-2013年间具有8个片段全基因组序列的B型流感病毒进行分析,利用Simplot软件筛选重配病毒,利用MEGA软件进行系统进化分析验证重配病毒.结果 获得432株B型流感病毒的全基因组序列,在66株代表病毒中,共计发现了22株重配株,其中9个为系内重配,13个为系间重配.在9个系内重配病毒中,2个B/Vic-87系内重配,7个B/Yam-88系内重配.Simplot软件结果和MEGA软件验证结果相似.结论 B型流感病毒间的重配事件频繁发生,既存在系内重配,也存在两系间的重配.Simplot软件可以用于快速筛选B型流感重配病毒.

Evolution and reassortment of influenza B virus during 1983-2013

Objective To study the evolution and reassortment of influenza B virus during 1983-2013.Methods Influenza B viruses with full genome sequence from 1983-2013 were downloaded from GenBank database for analysis.The reassortments of influenza B viruses were selected by Simplot program and were further analyzed and evaluated by MEGA program.Results In total 432 influenza B viruses had full genome sequences available for analysis,and among the 66 representative viruses,22 reassortant viruses were identified.Nine of the viruses were intra-lineage reassortants including 2 B/Vic-87 lineage viruses and 7 B/Yam-88 lineage viruses,while the other 13 were inter-lineage reassortants between B/Vic-87 and B/ Yam-88 lineages.Analysis with simplot program revealed similar result with MEGA program.Conclusions Reassortment of influenza B virus between different lineages or within the same lineage is frequent.Simplot program can be used for rapid screening of type B influenza reassortant virus.