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| EGF对瘢痕成纤维细胞影响的实验研究 | |
| 引用本文： | 赵娴,刘流,袁瑞红,赵德萍,孙家平,赫佳. EGF对瘢痕成纤维细胞影响的实验研究[J]. 皮肤病与性病, 2008, 30(4) |
| 作者姓名： | 赵娴 刘流 袁瑞红 赵德萍 孙家平 赫佳 |
| 作者单位： | 昆明医学院第一附属医院整形外科,云南,昆明,650032;昆明医学院第一附属医院临床医学实验研究中心,云南,昆明,650032 |
| 基金项目： | 云南省自然科学基金 ， 云南省社会发展攻关项目 |
| 摘 要： | 目的 通过探讨细胞因子EGF对瘢痕成纤维细胞生物学行为的影响,研究EGF在瘢痕形成中的作用机制,为临床预防和治疗瘢痕提供分子学水平及病理学水平的理论依据.方法 采用倒置显微镜、MTT测定、流式细胞术,对瘢痕成纤维细胞及正常皮肤成纤维细胞进行体外培养,观察、分析细胞因子EGF对瘢痕成纤维细胞生物学行为的影响.结果 倒置显微镜、流式细胞术以及MTT对体外培养瘢痕成纤维细胞和正常皮肤成纤维细胞的测定结果显示,与相应的对照组相比,加入细胞因子EGF后,体外培养的瘢痕成纤维细胞和正常皮肤成纤维细胞均明显增殖,且凋亡水平降低,显示EGF对成纤维细胞起着明显的促增殖作用.结论 细胞因子EGF对成纤维细胞的生长趋势与增殖活性有明显的促进作用,刺激了胶原纤维的合成、增生和沉积.因此,EGF可促进创伤的早期修复,但EGF的过量应用有可能导致增生性瘢痕的形成. |
| 关 键 词： | 瘢痕 细胞因子 成纤维细胞 创伤愈合 |
Effects of EGF on Scars Fibroblasts |
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| ZHAO Xian,LIU Liu,YUAN Rui-hong,ZHAO De-ping,SUN Jia-ping,HE Jia. Effects of EGF on Scars Fibroblasts[J]. Journal of Dermatology and Venereology, 2008, 30(4) | |
| Authors: | ZHAO Xian LIU Liu YUAN Rui-hong ZHAO De-ping SUN Jia-ping HE Jia |
| Affiliation: | ZHAO Xian1,LIU Liu1,YUAN Rui-hong1,ZHAO De-ping2,SUN Jia-ping1,HE Jia1(1. Department of Plastic Surgery,The First Affiliated Hospital of Kunrning Medical College,2. Center Of Clinical Medicine Empirical Study) |
| Abstract: | Objective To explore the mechanism of cytokines EGF for the scars,and to provide molecular and pathology theory for prevention and treatment of scars.Materials and Methods Fibroblasts of scars and normal skins were cultured in vitro. The results were observed and analyzed by light inverted microscope(LM),3-(4,5-dimethyl-thiazo l-2-yl)-2,5ciphenyltetrazolium bromi -de (MTT) assay,flow cytometry (FCM) to determine the effect of EGF on biological behavior of scars.Results In vitro:the results were observed by ... |
| Keywords: | Scars Cytokine Fibroblasts Wound healing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |