豚鼠冲击波负压暴露后耳蜗毛细胞扫描电镜和透射电镜观察

目的 探讨冲击波负压(BUP)暴露后豚鼠耳蜗毛细胞的超微结构变化特点.方法 将豚鼠暴露于不同强度的实验性BUP 1次,8h～7d后应用扫描电镜和透射电镜技术观察耳蜗基底膜毛细胞的超微结构变化.结果 负压峰值为-22.4 ～-63.3kPa的BUP暴露后,豚鼠耳蜗毛细胞的超微结构变化发生了不同程度的病理性改变,主要表现为第2、3排外毛细胞散在性缺失,甚至节段性外毛细胞缺失、纤毛融合甚至巨纤毛形成及胞浆溢出,纤毛内微丝解聚、线粒体肿胀、溶酶体数量增多和胞浆内空泡形成.结论 不同强度的BUP暴露可导致豚鼠耳蜗毛细胞的超微结构病变,且BUP强度越高,病变越重.

Scanning and transmission electron microscopic observation of cochlear hair cell injuries of guinea pig exposed to blast underpressure

Objective To explore ultrastructural characteristics of the cochlear hair cells in guinea pig exposed to blast underpressue(BUP).Methods The guinea pigs were killed 8h to 7d after exposure to experimental BUP,and their temporal bones were removed for fixation and ultrastructural observation using scanning and transmission electron microscope.Results The ultrastructure of the cochlear hair cells in the guinea pigs appeared obvious damages following exposure to BUP at peak underpressure between-22.4kPa and-63.3kPa.The second and third rows of outer hair cells were more severely damaged than the first row of outer hair cells.The most significant findings in transmission electron microscopy were depolymerization of actin filiaments with stereocilla,swelling of submembraneous cistern.The cytoplasmic changes were various changes of mitochondria,formation of vacuoles,and irregularly arranged stereocilia.Conclusion The exposure to BUP may induce pathological changes of the OHCs ultrostructure in guinea pigs.And that,the higher the peak of underpressure,the severer these ultrastuuctural changes.