食管癌中hMSH2启动子区CpG岛过甲基化研究

目的]探讨hMSH2甲基化在食管癌发生发展中的作用。方法]应用实时荧光甲基化特异性聚合酶链反应(real-time MSP)分别检测130例食管癌患者癌组织及癌旁正常组织中hMSH2甲基化情况,并分析其与临床病理等参数及预后之间的关系。结果]在130例原发性食管癌组织中,hMSH2甲基化的有63例(48.5%),与之相对应的癌旁正常组织中甲基化的有18例(13.8%)(P<0.05)。hMSH2高甲基化分别与患者的年龄、肿瘤远处转移及不良预后有关(P<0.05),而与患者性别、淋巴结转移及临床分期等无关(P>0.05)。结论]hMSH2高甲基化是食管癌频发的分子事件,也是导致其错配修复功能缺陷的重要原因之一。hMSH2基因启动子区甲基化可能在老龄化及食管癌的发生发展过程中发挥重要作用,是一个潜在的预后相关因子。

Hypermethylation of CpG Island in Promoter Region of hMSH2 in Esophageal Cancer

Purpose] To study the function of hMSH2 gene promoter region 5’-CpG island methylation in carcinogenesis of esophageal cancer.Methods] The promoter region of hMSH2 5’-CpG island methylation was detected in 130 cases with esophageal cancer and adjacent normal tissues by real-time methylation-specific PCR(real-time MSP),and its relationship with clinicopathological parameters and prognosis was analyzed.Results] In 130 cases with primary esophageal cancer,63(48.5%) cases occurred hMSH2 gene methylation in promoter region 5’-CpG island,cancer adjacent tissue methylation was found in 18(13.8%) cases,with statistically significant difference(P<0.05).hMSH2 gene promoter 5’-CpG island hypermethylation related to patients’age,distant metastasis and prognosis(P<0.05),but not to gender,lymph node metastasis and clinical stage(P>0.05).Conclusion] hMSH2 gene promoter region 5’-CpG island methylation is a frequent molecular event in esophageal cancer,and is one of the important reasons of causing defective mismatch repair.It may be play an important role in aging and carcinogenesis for esophageal cancer,and may be a potential prognostic factor.